The Glucoamylase cDNA from Aspergillus oryzae : Its Cloning, Nucleotide Sequence, and Expression in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
A cDNA for Aspergillus oryzae glucoamylase was cloned, using oligodeoxyribonucleotide probes derived from amino sequences of peptide fragments of the enzyme. The glucoamylase cDNA, when introduced into Saccharomyces cerevisiae, directed the secretion of active glucoamylase into the culture medium. The complete nucleotide sequence of the cDNA contained an open reading frame encoding 612 amino acid residues. Comparative studies with other fungal glucoamylases showed homologies of 67% with A. niger and 30% with Rhizopus oryzae of the deduced amino acid sequences. In the five conserved regions reported in other fungal glucoamylases, the levels of homologies between those regions of A. oryzae and A. niger enzymes were much higher (78-94%). A. oryzae glucoamylase contained no peptide region abundant in threonine and serine residue (TS-region), like that proposed to adsorb onto raw starch in A. awamori var. kawachii glucoamylase.
- 社団法人日本農芸化学会の論文
- 1991-04-23
著者
-
Hata Yoji
Research Institute, Gekkeikan Sake Co. Ltd.
-
Kitamoto K
Univ. Tokyo Tokyo Jpn
-
Kitamoto K
National Research Institute Of Brewing
-
Tamura G
Department Of Agricultural Chemistry The University Of Tokyo
-
Tamura G
Research Institute Of Brewing Resources Co. Ltd.
-
Tamura Gakuzo
Research Institute Of Brewing Resources Co. Ltd.
-
Hata Yoji
Research Institute Of Brewing Resources Co. Ltd.
-
Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
-
Kitamoto Katsuhiko
National Research Institute Of Brewing
-
GOMI Katsuya
National Research Institute of Brewing
-
HARA Shodo
National Research Institute of Brewing
-
Tamura Gakuzo
Nuclear Receptor Institute Ksp
-
KUMAGAI Chieko
National Research Institute of Brewing
-
Hata Yoji
Research Institite Of Gekkeikan Gekkeikan Sake Co. Ltd.
-
Kumagai C
National Research Institute Of Brewing
-
Hara S
General Research Laboratory Of Kiku-masamune Sake Brewing Co. Ltd.
-
Hara Shodo
Office Of Technical Officers Osaka Regional Taxation Bureau
-
Gomi K
Tohoku Univ. Miyagi Jpn
-
Kumagai Chieko
General Research Laboratories Ozeki Corp.
-
Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
関連論文
- Enhancement of β-glucosidase activity on the cell-surface of sake yeast by disruption of SED1(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- S19159, a Modulator of Neurite Outgrowth Produced by the Ascomycete Preussia aemulans II. Structure Elucidation
- Multidrug Resistance Phenotype Conferred by Overexpressing bfr2^+/pad1^+/sks1^+or pap1^+Genes and Mediated by bfr1^+Gene Product, a Structural and Functional Homologue of P-Glycoprotein in Schizosaccharomyces pombe
- Enhancement of β-glucosidase activity on the cell-surface of sake yeast by disruption of SED1
- Development of a Versatile Expression Plasmid Construction System for Aspergillus oryzae and Its Application to Visualization of Mitochondria
- Adenine Auxotrophic Mutants of Aspergillus oryzae : Development of a Novel Transformation System with Triple Auxotrophic Hosts
- Identification and Characterization of Scpl5, a Protein from Streptomyces coelicolor A3(2) Inducing Neurites in PC12 Cells(Biochemistry & Molecular Biology)
- Functional Expression in Aspergillus oryzae of p15, a Protein with Potent Neurite-inducing Activity in PC12 Cells(Microbiology & Fermentation Technology)
- Characterization of a Novel Fungal Protein, p15, Which Induces Neuronal Differentiation of PC12 Cells
- S19159, a Modulator of Neurite Outgrowth Produced by the Ascomycete Preussia aemulans I. Producing Strain, Fermentation, Isolation and Biological Activity
- Identification and Characterization of Porcine NP-190, a Novel Protein That Is Specifically Expressed in the Axonal Membrane during the Embryonic Period^1
- Isolation and Characterization of a 230 kDa Protein(p230) Specifically Expressed in Fetal Brains: Its Involvement in Neurite Outgrowth from Rat Cerebral Cortex Neurons Grown on Monolayer of Astrocytes
- Hop-Resistant Lactobacillus brevis Contains a Novel Plasmid Harboring a Multidrug Resistance-Like Gene
- The Gene Responsible for Tunicamycin Resistance, tmrB, in Bacillus subtilis(Microbilolgy & Fermentation Industry)
- 3E10-5 Effect of disruption of the Aopexll genes involved in peroxisome biogenesis on Woronin body formation in Aspergillus oryzae
- Development of a Modified Positive Selection Medium That Allows to Isolate Aspergillus oryzae Strains Cured of the Integrated niaD-Based Plasmid
- Visualizing Nuclear Migration during Conidiophore Development in Aspergillus nidulans and Aspergillus oryzae : Multinucleation of Conidia Occurs through Direct Migration of Plural Nuclei from Phialides and Confers Greater Viability and Early Germination i
- Effects of Protein Transport Inhibitors on the Distribution and Secretion of the Fusion Protein RntA-EGFP in Aspergillus oryzae
- 2F14-4 Visualization and structural analysis of septal pore in Aspergillus oryzaev
- In vivo Visualization of the Distribution of a Secretory Protein in Aspergillus oryzae Hyphae Using the RntA-EGFP Fusion Protein(Microbiology & Fermentation Technology)
- Visualization of Nuclei in Aspergillus oryzae with EGFP and Analysis of the Number of Nuclei in Each Conidium by FACS(Microbiology & Fermentation Technology)
- Production and Product Quality Assessment of Human Hepatitis B Virus Pre-S2 Antigen in Submerged and Solid-State Cultures of Aspergillus oryzae
- Brefeldin A, a Specific Inhibitor of Intracellular Translocation of Vesicular Stomatitis Virus G Protein: Intracellular Accumulation of High-mannose Type G Protein and Inhibition of Its Cell Surface Expression
- Stereochemical Configuration of 1-1 Glycosidic Linkages in Tunicamycin
- Effect of Brefeldin A on Biosynthesis of Cellular Components in Candida albicans
- A New Screening Procedure on Selective Inhibitors against SV40-Transformed Cells
- PPARγ Activation and Adipocyte Differentiation Induced by AS-6, a Prenyl-phenol Antidiabetic Antibiotic
- Induction of Two Types of Gene Amplification by a Cloned DNA Fragment and Amplification of a Foreign Gene on the Bacillus subtilis Chromosome by Using the Fragment
- Construction of New ARSI Vectors with Increased Mitotic Stability in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- Mutational Site in Tunicamycin-resistant Mutation tmrB8 of Bacillus subtilis as Revealed at the Nucleotide Sequence Level(Microbiology & Fermentation Industry)
- Efficacies of Different Secretion Vectors for Secretion of Human Epidermal Growth Factor by Escherichia coli(Biological Chemistry)
- Novel Saccharomyces cerevisiae Temperature-sensitive cyrl Mutant Which Accumulates Glycogen Particles
- recE-Dependent Gene Amplification Induced by a Tunicamycin-resistant Mutation (tmrA7) in Bacillus subtilis(Microbiology & Fermentation Industry)
- Expression and Secretion of Human Epidermal Growth Factor in Escherichia coli(Microbiology & Fermentation Industry)
- Sequence of the Signal Peptide of E. coli Alkaline Phosphatase
- Brewing Properties of Sake Yeast Whose EST2 Gene Encoding Isoamyl Acetate-Hydrolyzing Esterase Was Disrupted
- Molecular Cloning and Nucleotide Sequence of the Isoamyl Acetate-Hydrolyzing Esterase Gene (EST2) from Saccharomyces cerevisiae : The Mechanism of Isoamyl Acetate Production by Sake Yeast (IV)
- Direct Ethanol Production from Barley β-Glucan by Sake Yeast Displaying Aspergillus oryzae β-Glucosidase and Endoglucanase(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Cloning and Enhanced Expression of the Cytochrome P450nor Gene (nicA ; CYP55A5) Encoding Nitric Oxide Reductase from Aspergillus oryzae
- Construction of Uracil and Tryptophan Auxotrophic Mutants from Sake Yeasts by Disruption of URA3 and TRP1 Genes(Microbiology & Fermentation Industry)
- Cloning and Overexpression of β-N-Acetylglucosaminidase Encoding Gene nagA from Aspergillus oryzae and Enzyme-catalyzed Synthesis of Human Milk Oligosaccharide(Microbiology & Fermentation Technology)
- Cloning and Characterization of the nagA Gene that Encodes β-N-Acetylglucosaminidase from Aspergillus nidulans and Its Expression in Aspergillus oryzae(Microbiology & Fermentation Technology)
- A Novel Amine Oxidase-Encoding Gene from Aspergillus oryzae(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Isolation and Characterization of a Novel Gene Encoding α-L-Arabinofuranosidase from Aspergillus oryzae(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Cloning of a Novel Tyrosinase-Encoding Gene (melB) from Aspergillus oryzae and Its Overexpression in Solid-State Culture (Rice Koji)(BREWING AND FOOD TECHNOLOGY)
- The construction and application of diploid sake yeast with a homozygous mutation in the FAS2 gene(BREWING AND FOOD TECHNOLOGY)
- Effect of Yeast Fumarase Gene (FUM1) Dsruption on Production of Malic, Fumaric and Succinic Acids in Sake Mash
- Molecular Cloning and Determination of the Nucleotide Sequence of a Gene Encoding an Acid-Stable α-Amylase from Aspergillus kawachii
- Construction of Urea Non-producing Yeast Saccharomyces cerevisiae by Disruption of the CAR1 Gene(Microbiology & Fermentation Industry)
- Differentiation of Mouse and Human Myeloid Leukemia Cells Induced by an Antitumor Antibiotic, Ascofuranone(Biological Chemistry)
- Insertion Analysis of Putative Functional Elements in the Promoter Region of the Aspergillus oryzae Taka-amylase A Gene (amyB) Using a Heterologous Aspergillus nidulans amdS-lacZ Fusion Gene System
- Characteristic Expression of Three Amylase-encoding Genes, agdA, amyB, and glaA in Aspergillus oryzae Transformants Containing Multiple Copies of the agdA Gene
- Nucleotide Sequence and Expression of α-GIucosidase-encoding Gene (agdA) from Aspergillus oryzae
- Cloning and Nucleotide Sequence of the Calmodulin-Encoding Gene (cmdA) from Aspergillus oryzae
- High Level Secretion of Calf Chymosin Using a Glucoamylase-prochymosin Fusion Gene in Aspergillus oryzae
- Deletion Analysis of the Taka-amylase A Gene Promoter Using a Homologous Transformation System in Aspergillus oryzae
- Identification of the Promoter Region of the Taka-amylase A Gene Required for Starch Induction(Microbiology & Fermentation Industry)
- The Glucoamylase cDNA from Aspergillus oryzae : Its Cloning, Nucleotide Sequence, and Expression in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- Rapid Assay of Glucoamylase Using a Fluorescence-labeled Glucoamylase Inhibitor, Acarbose
- Chromosomal Transformation in Saccharomyces cerevisiae with DNA Isolated by Pulse Field Gel Electrophoresis(Microbiology & Fermentation Industry)
- Overproduction of an α-Amylase/Glucoamylase Fusion Protein in Aspergillus oryzae Using a High Expression Vector
- Construction of an α-Amylase/Glucoamylase Fusion Gene and Its Expression in Saccharomyces cerevisiae
- Characteristics of Koji Prepared from the Transformant of Aspergillus oryzae with the Glucoamylase Gene of Aspergillus shirousamii, and Its Utilization for Sake-Brewing
- Cloning of the α-Amylase cDNA of Aspergillus shirousamii and Its Expression in Saccharomyces cerevisiae
- Immunological Comparison of Nitrite Reductase from Different Plant Sources
- Electron Donor for Nitrite Reductase from Etiolated Bean Stems
- Some Properties of Ferredoxin-nitrite Reductase from Spinacia oleracea
- Characterization of Substances that Restore Impaired Cell Division of UV-Irradiated E. coli B
- Antitumor Activity of 5-Hydroxymethyl-2-furoic Acid
- Functional Role of Aspergillus oryzae Glucoamylase C-Terminal Domain Investigated Using Its cDNA
- Using promoter replacement and selection for loss of heterozygosity to generate an industrially applicable sake yeast strain that homozygously overproduces isoamyl acetate(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Efficient and Direct Fermentation of Starch to Ethanol by Sake Yeast Strains Displaying Fungal Glucoamylases
- Molecular Cloning of the Glucoamylase Gene of Aspergillus shirousami and Its Expression in Aspergillus oryzae(Microbiology & Fermentation Industry)
- Cloning and Nucleotide Sequence of the Genomic Taka-amylase A Gene of Aspergillus oryzae(Microbiology & Fermentation Industry)
- Cloning and Nucleotide Sequence of the Ribonuclease T_1 Gene (rntA) from Aspergillus oryzae and Its Expression in Saccharomyces cerevisiae and Aspergillus oryzae
- BR-P18 Development of novel cell-surface display system of Aspergillus oryzae using chitin binding domain(Section IV Biorefinery)
- Functional Analysis of Genes Encoding Putative Oxidoreductases in Aspergillus oryzae, Which Are Similar to Fungal Fructosyl-Amino Acid Oxidase(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Molecular Cloning and Characterization of a Transcriptional Activator Gene, amyR, Involved in the Amylolytic Gene Expression in Aspergillus oryzae
- Molecular Cloning of a Genomic DNA for Enolase from Aspergillus oryzae
- Deletion Analysis of the Catalase-Encoding Gene (catB) Promoter from Aspergillus oryzae
- Identification and Characterization of an Intracellular Lectin, Calnexin, from Aspergillus oryzae Using N-Glycan-Conjugated Beads
- Regulation of the Glucoamylase-Encoding Gene(glaB), Expressed in Solid-State Culture(Koji)of Aspergillus oryzae
- Comparision of Two Glucoamylases Produced by Aspergillus oryzae in Solid-State Culture (Koji) and in Submerged Culture
- Breeding of a Sake Yeast with Improved Ethyl Caproate Productivity(Microbiology & Fermentation Industry)
- Cloning and Functional Analysis of the Aspergillus oryzae Conidiation Regulator Gene brlA by Its Disruption and Misscheduled Expression
- 904 Isolation and Characterization of Mutants of Saccharomyces cerevisiae with pH-sensitive Growth
- Breeding of a New Type of Baker's Yeast by δ-Integration for Overoproduction of Glucoamylase Using a Homothallic Yeast
- Electrophoretic Karyotype and Gene Assignment to Chromosomes of Aspergillus oryzae
- A Novel Culture Method for High Level Production of Heterologous Protein in Saccharomyces cerevisiae
- Cloning and Nucleotide Sequence of the Acid Protease-encoding Gene (pepA) from Aspergillus oryzae
- Mutant Isolation of Non-Urea Producing Sake Yeast by Positive Selection
- Transformation of the Industrial Strain of Aspergillus oryzae with the Homologous amdS Gene as a Dominant Selectable Marker
- Site-directed Mutagenesis of Catalytic Active-site Residues of Taka-amylase A
- Improvement of Heterologous Protein Production in Aspergillus oryzae by RNA Interference with α-Amylase Genes
- Efficient and Direct Fermentation of Starch to Ethanol by Sake Yeast Strains Displaying Fungal Glucoamylases
- The Glucoamylase-Encoding Gene (glaB) Is Expressed in Solid-State Culture with A Low Water Content
- Production of Fungal Cell Wall Lytic Enzymes by Oerskovia sp. CK and Some Propertics of the Crude Enzymes.
- Transformation System for Aspergillus oryzae with Double Auxotrophic Mutations, niaD and sC
- Transformation of Aspergillus oryzae through Plasmid-mediated Complementation of the Methionine-auxotrophic Mutation(Microbiology & Fermentation Industry)
- Identification of a Heat-Stable Precursor of Dimethyl Sulfide in Sake