Characterization of Substances that Restore Impaired Cell Division of UV-Irradiated E. coli B
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概要
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Substances which restore impaired cell division in UV-irradiated E. coli B were surveyed among various bacteria. The active substance was found only in several genera of Gram-negative bacteria, i.e., Escherichia, Enterobacter, Salmonella and some species of Pseudomonas. The activity in the dialyzed cell extract of E. coli B/r was observed in the presence of β-NAD and was enhanced by Mg2+ and Mn2+. The active substance was very labile, but the activity was protected by 1 mM dithiothreitol in the process of purification. The activity of a fraction recovered through DEAE-cellulose column chromatography was stimulated by the presence of membrane fraction. Upon treatment with lipid-degrading enzymes and proteases, the division-stimulating activity was lost or reduced. It appears that the inactivation by lipase and phospholipase A2 was due to the formation of lysophospholipids and that a proteinous substance participated in the recovery of impaired cell division of UV-irradiated E. coli B.
- 社団法人 日本農芸化学会の論文
著者
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TAMURA Gakuzo
Department of Agricultural Chemistry, University of Tokyo
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Tamura G
Department Of Agricultural Chemistry The University Of Tokyo
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Tamura Gakuzo
Depart Ment Qf Agricultural Chemistry The University Of Tokyo
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YOSHIYAMA Yoshihiro
Department of Agricultural Chemistry, The University of Tokyo
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SHIMOII Hitoshi
Department of Agricultural Chemistry, The University of Tokyo
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TAMURA Gakuzo
Department of Agricultural Chemistry, The University of Tokyo
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