Some Properties of Ferredoxin-nitrite Reductase from Spinacia oleracea
スポンサーリンク
概要
- 論文の詳細を見る
Ferredoxin-nitrite reductase (EC 1.7.7. 1), an enzyme which catalyzes the 6-electron reduction of nitrite to ammonia, has been isolated from Spinacia oleracea. The isolated enzyme was homogeneous by disc electrophoresis with polyacrylamide gel. The molecular weight of the enzyme was estimated to be 86, 000 by Ultrogel AcA 34 gel filtration. In the oxidized form, the enzyme had absorption maxima at 278, 388 (Soret band), 573 (α band) and 690nm, indicating that siroheme is directly involved in the catalysis of nitrite reduction. This absorption spectrum was modified by sulfite, hydroxylamine and cyanide. The enzyme exhibited electron paramagnetic resonance signals with g values of 6.9 and 5.2, which are characteristic ofa high spin Fe3+-siroheme in the molecule. These signals disappeared upon the addition of dithionite or nitrite. This isolated enzyme also contained four moles of labile sulfide and 7g-atoms of iron per 86, 000g of protein.
- 社団法人 日本農芸化学会の論文
著者
-
Tamura G
Department Of Agricultural Chemistry The University Of Tokyo
-
TAMURA Goro
Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University
-
HIRASAWA-SOGA Masakazu
Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University
-
Tamura Goro
Department Of Agricultural Chemistry Faclty Of Horticulture Chiba University
関連論文
- The Gene Responsible for Tunicamycin Resistance, tmrB, in Bacillus subtilis(Microbilolgy & Fermentation Industry)
- Brefeldin A, a Specific Inhibitor of Intracellular Translocation of Vesicular Stomatitis Virus G Protein: Intracellular Accumulation of High-mannose Type G Protein and Inhibition of Its Cell Surface Expression
- Stereochemical Configuration of 1-1 Glycosidic Linkages in Tunicamycin
- Effect of Brefeldin A on Biosynthesis of Cellular Components in Candida albicans
- A New Screening Procedure on Selective Inhibitors against SV40-Transformed Cells
- PPARγ Activation and Adipocyte Differentiation Induced by AS-6, a Prenyl-phenol Antidiabetic Antibiotic
- Induction of Two Types of Gene Amplification by a Cloned DNA Fragment and Amplification of a Foreign Gene on the Bacillus subtilis Chromosome by Using the Fragment
- Construction of New ARSI Vectors with Increased Mitotic Stability in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- Mutational Site in Tunicamycin-resistant Mutation tmrB8 of Bacillus subtilis as Revealed at the Nucleotide Sequence Level(Microbiology & Fermentation Industry)
- Efficacies of Different Secretion Vectors for Secretion of Human Epidermal Growth Factor by Escherichia coli(Biological Chemistry)
- Novel Saccharomyces cerevisiae Temperature-sensitive cyrl Mutant Which Accumulates Glycogen Particles
- recE-Dependent Gene Amplification Induced by a Tunicamycin-resistant Mutation (tmrA7) in Bacillus subtilis(Microbiology & Fermentation Industry)
- Expression and Secretion of Human Epidermal Growth Factor in Escherichia coli(Microbiology & Fermentation Industry)
- Sequence of the Signal Peptide of E. coli Alkaline Phosphatase
- Construction of Urea Non-producing Yeast Saccharomyces cerevisiae by Disruption of the CAR1 Gene(Microbiology & Fermentation Industry)
- Differentiation of Mouse and Human Myeloid Leukemia Cells Induced by an Antitumor Antibiotic, Ascofuranone(Biological Chemistry)
- Insertion Analysis of Putative Functional Elements in the Promoter Region of the Aspergillus oryzae Taka-amylase A Gene (amyB) Using a Heterologous Aspergillus nidulans amdS-lacZ Fusion Gene System
- Characteristic Expression of Three Amylase-encoding Genes, agdA, amyB, and glaA in Aspergillus oryzae Transformants Containing Multiple Copies of the agdA Gene
- Nucleotide Sequence and Expression of α-GIucosidase-encoding Gene (agdA) from Aspergillus oryzae
- Cloning and Nucleotide Sequence of the Calmodulin-Encoding Gene (cmdA) from Aspergillus oryzae
- High Level Secretion of Calf Chymosin Using a Glucoamylase-prochymosin Fusion Gene in Aspergillus oryzae
- Deletion Analysis of the Taka-amylase A Gene Promoter Using a Homologous Transformation System in Aspergillus oryzae
- Identification of the Promoter Region of the Taka-amylase A Gene Required for Starch Induction(Microbiology & Fermentation Industry)
- The Glucoamylase cDNA from Aspergillus oryzae : Its Cloning, Nucleotide Sequence, and Expression in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- Overproduction of an α-Amylase/Glucoamylase Fusion Protein in Aspergillus oryzae Using a High Expression Vector
- Construction of an α-Amylase/Glucoamylase Fusion Gene and Its Expression in Saccharomyces cerevisiae
- Characteristics of Koji Prepared from the Transformant of Aspergillus oryzae with the Glucoamylase Gene of Aspergillus shirousamii, and Its Utilization for Sake-Brewing
- Cloning of the α-Amylase cDNA of Aspergillus shirousamii and Its Expression in Saccharomyces cerevisiae
- Immunological Comparison of Nitrite Reductase from Different Plant Sources
- Electron Donor for Nitrite Reductase from Etiolated Bean Stems
- Some Properties of Ferredoxin-nitrite Reductase from Spinacia oleracea
- Characterization of Substances that Restore Impaired Cell Division of UV-Irradiated E. coli B
- Antitumor Activity of 5-Hydroxymethyl-2-furoic Acid
- Purification and Some Properties of Plant Serine Acetyltransferase
- Measurement of Serine Acetyltransferase Activity in Crude Plant Extracts by a Coupled Assay System Using Cysteine Synthase
- Molecular Cloning of the Glucoamylase Gene of Aspergillus shirousami and Its Expression in Aspergillus oryzae(Microbiology & Fermentation Industry)
- Purification and Characterization of Ferredoxin-Sulfite Reductase from Turnip (Brassica rapa) Leaves and Comparison of Properties with Ferredoxin-Sulfite Reductase from Turnip Roots
- Effect of Sulfur and Nitrogen Nutrition on Derepression of Ferredoxin-Sulfite Reductase in Leek Seedlings
- Inhibitory Effects of a Fermented "Makomo" Leaf Extract on Insoluble-glucan Synthesis by Serotype c Streptococcus mutans Glucosyltransferases
- Isolation and Partial Characterization of Homogeneous Nitrite Reductase from a Cyanobacterium, Aphanothece sacrum
- Isolation of Serine Acetyltransferase Complexed with Cysteine Synthase from Allium tuberosum(Biological Chemistry)
- Five Isoforms of Cysteine Synthase in Rape Leaves(Biological Chemistry)
- Reduction of S-Sulfoglutathione by Cyanobacterial Ferredoxin-sulfite Reductase(Biological Chemistry)
- Ferredoxin-sulfite Reductase from a Cyanobacterium, Spirulina platensis(Biological Chemistry)
- Isolation and Partial Characterization of Homogeneous Ferredoxin-sulfite Reductase from a Red Alga, Porphyra yezoensis(Biological Chemistry)
- Purification and Partial Characterization of Ferredoxinsulfite Reductase from Rape Leaves(Biological Chemistry)
- Purification and Characterization of Ferredoxin-Sulfite Reductases from Leek(Allium tuberosum) Leaves
- Ferredoxin isoforms from Chlorella vulgaris (Chlorococcales, Chlorophyceae): Molecular characterization and participation in ferredoxin-dependent enzymes
- Isolation and Partial Characterization of Homogeneous Nitrite Reductase from a Red Alga, Porphyra yezoensis(Biological Chemistry)
- Nitrite Reductase in the Cyanobacterium Spirulina platensis
- Ferredoxin-linked Sulfite Reductase from Turnip Roots
- Further Characterization of Ferredoxin Nitrite Reductase and the Relationship between the Enzyme and Methyl Viologen-dependent Nitrite Reductase
- Methyl Viologen-linked Nitrite Reductase from Bean Roots