Effect of Brefeldin A on Biosynthesis of Cellular Components in Candida albicans
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概要
- 論文の詳細を見る
Brefeldin A (BFA) is an antibiotic having diverse biological effects such as antifungal, antiviral and antitumor activities. The effect of BFA on biosynthesis of cellular components was examined to elucidate the mode of action of BFA using C. albicans JAM 4888. When C. albicans was grown in the presence of BFA, cells became rounded and enlarged several times larger than the untreated control cells. Cell walls of the treated cells became irregular and a number of Sudan III-stainable lipid droplets was formed in the cytoplasm. Accompanying these morphological changes, a marked alteration occurred in the cellular lipid composition; neutral lipids increased whereas phospholipid decreased. [14C]Acetate incorporation into the lipid fraction proceeded in accordance with the growth in the presence of BFA. On the other hand, [32P]orthophosphate incorporation into phospholipid was severely inhibited. Incorporation of radiolabeled precursors into DNA, RNA and protein was not affected on a cell weight basis.
- 社団法人 日本農芸化学会の論文
著者
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Takatsuki A
Riken Inst. Physical And Chemical Res. Saitama Jpn
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Tamura G
Department Of Agricultural Chemistry The University Of Tokyo
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TAKATSUKI Akira
Laboratory of Microbiology, Department of Agricultural Chemistry, The University of Tokyo
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TAMURA Gakuzo
Laboratory of Microbiology, Department of Agricultural Chemistry, The University of Tokyo
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HAYASHI Toshiaki
Laboratory of Microbiology, Department of Agricultural Chemistry, Faculty of Agriculture, The Univer
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TAKATSUKI Akira
Laboratory of Microbiology, Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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TAMURA Gakuzo
Laboratory of Microbiology, Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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HAYASHI Toshiaki
Laboratory of Microbiology, Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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