Gene Amplification of the amy E-tmrB Region in Bacillus subtilis

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概要

• 論文の詳細を見る

• Bacillus subtilis B7, a tmrA mutant, shows both tunicamycin resistance and α-amylase hyperproductivity. The tmrA characters can be transferred simultaneously to recipient cells by DNA-mediated transformation. We found a typical gene amplification phenomenon in the tmrA transformants and B7 strain. The amplified unit, 16.3kb in size, covers α-amylase structural gene amyE to another tunicamycin resistance gene tmrB, which is located 9kb downstream of the amyE gene. About 10 repeating units are supposed to be tandemly repeated in the transformants. Amplification of the wild amyE and tmrB genes could be the cause of the α-amylase hyperproductivity and tunicamycin resistance of the tmrA transformants and B7 strain.

• 社団法人 日本農芸化学会の論文

著者

• YAMASAKI Makari

  Department of Agricultural Chemistry, the University of Tokyo

• YODA Koji

  Department of Biotechnology, University of Tokyo

• Yoda K

  Univ. Tokyo Tokyo Jpn

• Takatsuki A

  Riken Inst. Physical And Chemical Res. Saitama Jpn

• HARADA Shigeyoshi

  Department of Agricultural Chemistry, University of Tokyo

• MORI Masaki

  Department of Agricultural Chemistry, University of Tokyo

• TANIMOTO Akihiko

  Department of Agricultural Chemistry, University of Tokyo

• FURUSATO Takashi

  Institute of Biological Sciences, University of Tsukuba

• YAMANE Kunio

  Institute of Biological Sciences, University of Tsukuba

• TAKATSUKI Akira

  Department of Agricultural Chemistry, University of Tokyo

• TAMURA Gakuzo

  Department of Agricultural Chemistry, University of Tokyo

• HASHIGUCHI Ken-ichi

  Department of Agricultural Chemistry, The University of Tokyo

• NOMURA Shintaro

  Institute of Applied Microbiology, The University of Tokyo

• Yamane Kunio

  Institute Of Biological Sciences University Of Tsukuba

• Tamura Gakuzo

  Depart Ment Qf Agricultural Chemistry The University Of Tokyo

• Yoda Koji

  Department Of Agricultural Chemistry The University Of Tokyo

• Yamasaki Makari

  Department Of Agricultural Chemistry The University Of Tokyo

• Hashiguchi Ken-ichi

  Department Of Agricultural Chemistry The University Of Tokyo

• Tanimoto Akihiko

  Department Of Agricultural Chemistry The University Of Tokyo:(present Office)gyosei High School

• Harada Shigeyoshi

  Department Of Agricultural Chemistry The University Of Tokyo

• YODA Koji

  Department of Agricultural Chemistry, The University of Tokyo

• TAKATSUKI Akira

  Department of Agricultural Chemistry, The University of Tokyo

• YAMANE Kunio

  Institute of Applied Microbiology, The University of Tokyo

• TANIMOTO Akihiko

  Department of Agricultural Chemistry, The University of Tokyo

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