Hop-Resistant Lactobacillus brevis Contains a Novel Plasmid Harboring a Multidrug Resistance-Like Gene
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概要
- 論文の詳細を見る
The bitter-tasting compounds derived from the flowers of the hop plant (Humulus lupulus L.) protect beer from bacterial spoilage. However, a few lactic acid bacteria, especially lactobacilli, are resistant to these compounds and sometimes cause serious spoilage in the beer industry. It is importatnt to elecidate the mechanisms of hop-resistance in lactic acid bacteria. We selected mutants of Lactobacillus brevis resistant to high concentrations of the hop compounds. The parental strain, L. brevis ABBC45,carries several plasmids. The copy number of one plasmid, termed pRH45,was remarkably increased in one of the hop-resistant mutants compared with that in the wild-type strain. pRH45 (15.0 kb) contains an open reading frame of 1749 b, termed horA, the deduced protein of which includes six putative transmembrane domains and an ATP-binding domain. The amino acid sequence of this putative protein is significantly homologous to half molecules of a mammalian multidrug resistance gene product, P-glycoprotein, and to several bacterial ABC transporters. Furthermore, the hop-resistant mutant was found to be weakly resistant to novobiocin and ethidium bromide, which are structurally and functionally unrelated to the hop compounds. A possible role of the potential drug efflux pump gene in the hop-resistance of L. brevis is discussed.
- 社団法人日本生物工学会の論文
- 1997-07-25
著者
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Kitamoto Katsuhiko
Department of Biotechnology, The University of Tokyo
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Yamasaki M
Department Of Biotechnology The University Of Tokyo:(present Address)department Of Food Science And
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YAMASAKI Makari
Department of Agricultural Chemistry, the University of Tokyo
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YODA Koji
Department of Biotechnology, University of Tokyo
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Yoda K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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SAMI MANABU
Brewing Research & Development Laboratory, Asahi Breweries Ltd.,
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YAMASHITA HIROSHI
Brewing Research & Development Laboratory, Asahi Breweries Ltd.,
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HIRONO TATSUHIKO
Brewing Research & Development Laboratory, Asahi Breweries Ltd.,
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KADOKURA HIROSHI
Department of Biotechnology, The University of Tokyo
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Sami Manabu
Brewing Research & Development Laboratory Asahi Breweries Ltd.
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Yamane K
Univ. Tsukuba
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Yoda Koji
Department Of Biotechnology The University Of Tokyo
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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Yoda Koji
Department Of Agricultural Chemistry The University Of Tokyo
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Yamasaki Makari
Advanced Research Institute For The Science And Humanities Nihon University
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Yamasaki Makari
Department Of Agricultural Chemistry The University Of Tokyo
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Kadokura Hiroshi
Department Of Biotechnology The University Of Tokyo
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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Hirono Tatsuhiko
Brewing Research & Development Laboratory Asahi Breweries Ltd.
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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Yoda K
Institute Of Biological Sciences University Of Tsukuba
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Yamashita Hiroshi
Brewing Research & Development Laboratory Asahi Breweries Ltd.
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