The Effect of Pyruvate Decarboxylase Gene Knockout in Saccharomyces cerevisiae on L-Lactic Acid Production
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概要
- 論文の詳細を見る
A plant- and crop-based renewable plastic, poly-lactic acid (PLA), is receiving attention as a new material for a sustainable society in place of petroleum-based plastics. We constructed a metabolically engineered Saccharomyces cerevisiae that has both pyruvate decarboxylase genes (PDC1 and PDC5) disrupted in the genetic background to express two copies of the bovine L-lactate dehydrogenase (LDH) gene. With this recombinant, the yield of lactate was 82.3 g/liter, up to 81.5% of the glucose being transformed into lactic acid on neutralizing cultivation, although pdc1 pdc5 double disruption led to ineffective decreases in cell growth and fermentation speed. This strain showed lactate productivity improvement as much as 1.5 times higher than the previous strain. This production yield is the highest value for a lactic acid-producing yeast yet reported.
- 社団法人 日本農芸化学会の論文
- 2006-05-23
著者
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Kitamoto Katsuhiko
Department of Biotechnology, The University of Tokyo
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Nagamori Eiji
Department Of Biotechnology School Of Engineering Nagoya University
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Nagamori Eiji
Toyota Central R & D Labs. Inc.
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Nagamori E
Osaka Univ. Osaka Jpn
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Nagamori Eiji
Biotechnology Laboratory Toyota Central R&d Labs Inc.
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Kitamoto Katsuhiko
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Takahashi Haruo
Biotechnology Laboratory Toyota Central R&d Labs Inc.
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ISHIDA Nobuhiro
Biotechnology Laboratory, Toyota Central R&D Labs Inc.
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SAITOH Satoshi
Toyota Biotechnology & Afforestation Laboratory, Toyota Motor Co.
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ONISHI Toru
Toyota Biotechnology & Afforestation Laboratory, Toyota Motor Co.
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TOKUHIRO Kenro
Biotechnology Laboratory, Toyota Central R&D Labs Inc.
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Saitoh Satoshi
Toyota Biotechnology & Afforestation Laboratory Toyota Motor Co.
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Onishi Toru
Toyota Biotechnology & Afforestation Laboratory Toyota Motor Co.
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Ishida Nobuhiro
Biotechnology Laboratory Toyota Central R&d Labs Inc.
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Tokuhiro Kenro
Biotechnology Laboratory Toyota Central R&d Labs Inc.
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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Ishida Nobuhiro
Biotechnology Laboratoiy Toyota Central R&d Labs Inc.
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Takahashi Haruo
Biotechnology Laboratoiy, Toyota Central R&D Labs Inc.
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