Effects of Protein Transport Inhibitors on the Distribution and Secretion of the Fusion Protein RntA-EGFP in Aspergillus oryzae
スポンサーリンク
概要
- 論文の詳細を見る
The distribution of the secreted protein ribonuclease T1 (RntA) fused with the enhanced green fluorescent protein (EGFP), RntA-EGFP, was visualized in hyphae of Aspergillus oryzae in the presence of a protein transport inhibitor, brefeldin A, cytochalasin A, or nocodazole. During treatment with the protein transport inhibitors, the distribution of RntA-EGFP changed and distinct patterns of fluorescence accumulation were observed. The addition of brefeldin A caused RntA-EGFP fluorescence to appear in reticular networks, and the disruption of the polymerization of actin filaments by cytochalasin A caused an increase in RntA-EGFP fluorescence intensity in the hyphae without accumulation in a specific cellular component. In contrast, RntA-EGFP fluorescence was distributed in different parts of a hypha during treatment with nocodazole, a compound that depolymerizes microtubules. In addition, quantitative analysis was performed using the RntA-EGFP visualization system to analyze the relative amount of RntA-EGFP secreted into the culture medium during treatment with the protein transport inhibitors.
- 社団法人 日本農芸化学会の論文
- 2004-07-23
著者
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Kitamoto Katsuhiko
Department of Biotechnology, The University of Tokyo
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Nakajima Hiroo
京都府立医科大学 内分泌・乳腺外科学部門
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Nakajima Hiroo
Tsujigiwa Daigo Clinic 1-19 Daigo Shinmachi-uramachi Fushimi-ku Kyoto 601-1326 Jpn
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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Kitamoto Katsuhiko
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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MARUYAMA Jun-ichi
Department of Biotechnology, The University of Tokyo
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Maruyama J
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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NAKAJIMA Harushi
Department of Biotechnology, The University of Tokyo
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MASAI Kumiko
Department of Biotechnology, University of Tokyo
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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Maruyama Jun-ichi
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Kitamoto Katsuhiko
National Research Institute Of Brewing:(present Address)department Of Biotechnology The University O
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Nakajima H
Department Of Endocrine Breast Surgery Kyoto Prefectural University Of Medicine
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Masai Kumiko
Department Of Biotechnology University Of Tokyo
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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Maruyama Jun-ichi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Nakajima Harushi
Department Of Agricultural Chemistry The University Of Tokyo
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Nakajima Hidenori
Exploratory Research Laboratories Fujisawa Pharmaceutical Co. Ltd
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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Maruyama Junichi
Department Of Biotechnology The University Of Tokyo
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