Adenine Auxotrophic Mutants of Aspergillus oryzae : Development of a Novel Transformation System with Triple Auxotrophic Hosts
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概要
- 論文の詳細を見る
adeA and adeB genes homologous to Saccharomyces cerevisiae ADE1 and ADE2, respectively, were cloned from Aspergillus oryzae. AdeA and AdeB share 62.8% and 52.5% identities with S. cerevisiae Ade1 and Ade2, respectively. In order to obtain triple auxotrophic mutants from A. oryzae, 12 red-colored mutant colonies were isolated by UV mutagenesis of a double auxotrophic host, NS4 (niaD−, sC−), as a parent strain. All the mutants exhibited adenine auxotrophy and showed fluorescence in the vacuoles due to accumulation of a purine biosynthetic pathway precursor. Adenine auxotrophy of all the mutants was restored by introduction of either A. oryzae adeA or adeB genes. Sequence analysis demonstrated that substitutions or deletions of a single base pair occurred, inducing substitutions or frame shifts of amino acid sequences in both ade genes complementing the mutants. This study provides a novel host-vector system with triple auxotrophy in A. oryzae.
- 社団法人 日本農芸化学会の論文
- 2004-03-23
著者
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Kitamoto Katsuhiko
Department of Biotechnology, The University of Tokyo
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Arioka Manabu
Department Of Biotechnology University Of Tokyo
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Arioka M
Department Of Biotechnology The University Of Tokyo
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Arioka Manabu
東京大学
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Arioka Manabu
Department Of Biotechnology The University Of Tokyo
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Jin Feng
Noda Inst. Sci. Res.
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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Kitamoto Katsuhiko
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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MARUYAMA Jun-ichi
Department of Biotechnology, The University of Tokyo
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JIN Feng
Department of Biotechnology, The University of Tokyo
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JUVVADI Praveen
Department of Biotechnology, The University of Tokyo
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Maruyama J
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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JUVVADI Praveen
Dept. Biotech., Univ. Tokyo
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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Maruyama Jun-ichi
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
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Kitamoto Katsuhiko
National Research Institute Of Brewing:(present Address)department Of Biotechnology The University O
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Jin Feng
Department Of Anthropology The University Of Tokyo
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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Juvvadi Praveen
Department Of Biotechnology The University Of Tokyo
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Maruyama Jun-ichi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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Maruyama Junichi
Department Of Biotechnology The University Of Tokyo
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