Characterization of a Novel Fungal Protein, p15, Which Induces Neuronal Differentiation of PC12 Cells.
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概要
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In our previous paper, we reported that a 15 kDa protein (p15) produced by a fungus, genus Helicosporium, enhanced NGF-induced neurite outgrowth from PC12 cells. Here we further characterized the actions of p15. The complete amino acid sequence of p15 was determined and it was shown to be a hydrophilic protein composed of 118 amino acid residues with two intramolecular disulfide bridges. p15-induced neurite outgrowth was blocked by the depletion of extracellular Ca2+ in the culture medium and was significantly inhibited by L-type Ca2+ channel inhibitor nicardipine. p15 stimulated Src kinase and MAPK activities, and neurite outgrowth was not observed in sreDN2, a dominant negative c-src-express-ing cell line, and was significantly reduced in RasN17-expressing cells. These results suggest that p15 stimulates neurite outgrowth through the potentiation of L-type Ca2+-channels, thereby activating the Src-Ras-MAPK cascade.
- 社団法人 日本生化学会の論文
著者
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Arioka Manabu
Department Of Biotechnology The University Of Tokyo
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Wakatsuki Shuji
Department Of Biotechnology University Of Tokyo:(present Address)department Of Growth Regulation Ins
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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Dohmae Naoshi
Diuisian of Biomolecular Characterization, The Institute of Physical and Chemical Researcli (RIKFN)
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Takio Koji
Diuisian of Biomolecular Characterization, The Institute of Physical and Chemical Researcli (RIKFN)
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Yamasaki Makari
College of Bioresource Sciences, Department of Food Science and Technology, Nihon Unicersity
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Yamasaki Makari
College of Bioresource Science, Department of Food Science and Technology, Nihon University
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