Visualization of Nuclei in Aspergillus oryzae with EGFP and Analysis of the Number of Nuclei in Each Conidium by FACS(Microbiology & Fermentation Technology)

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概要

• 論文の詳細を見る

• Aspergillus oryzae has been reported to form conidia with multinuclei. In order to analyze nuclei in living cells, we developed an expression system of the A. nidulans histone H2B protein tagged by EGFP (H2B : : EGFP). In both A. oryzae niaD300 and A. nidulans FGSC89 transformants expressing H2B : : EGFP, fluorescence was detected in nuclear regions of hyphae and conidia. While a conidium contained only one fluorescent spot in the A. nidulans transformant, approximately 66% of conidia had two, 24% had one, and 10% had three or more in the A. oryzae transformant. The conidia expressing H2B : : EGFP were put through FACS (fluorescence-activated cell sorting) analysis and two sharp peaks, corresponding to one and two nuclei in each conidium, were noted in the A. oryzae transformant. In addition, the A. oryzae uninucleate conidia that were successfully isolated by FACS reproduced conidia with almost the same number distribution of nuclei as that of the original. Conidia of five A. oryzae strains used in sake brewing were scored for the number of nuclei, showing that a varied number of nuclei existed in each conidium and some strains had a small number of uninucleate conidia.

• 社団法人日本農芸化学会の論文
• 2001-07-23

著者

• Kitamoto Katsuhiko

  Department of Biotechnology, The University of Tokyo

• Nakajima Hiroo

  京都府立医科大学 内分泌･乳腺外科学部門

• Nakajima Hiroo

  Tsujigiwa Daigo Clinic 1-19 Daigo Shinmachi-uramachi Fushimi-ku Kyoto 601-1326 Jpn

• Kitamoto K

  Univ. Tokyo Tokyo Jpn

• Kitamoto K

  National Research Institute Of Brewing

• Kitamoto Katsuhiko

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• MARUYAMA Jun-ichi

  Department of Biotechnology, The University of Tokyo

• Maruyama J

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• NAKAJIMA Harushi

  Department of Biotechnology, The University of Tokyo

• Kitamoto Katsuhiko

  Department Of Biotechnology University Of Tokyo

• Maruyama Jun-ichi

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo

• Kitamoto Katsuhiko

  National Research Institute Of Brewing:(present Address)department Of Biotechnology The University O

• Nakajima H

  Department Of Endocrine Breast Surgery Kyoto Prefectural University Of Medicine

• Kitamoto Katsuhiko

  Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate

• Maruyama Jun-ichi

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Nakajima Harushi

  Department Of Agricultural Chemistry The University Of Tokyo

• Nakajima Hidenori

  Exploratory Research Laboratories Fujisawa Pharmaceutical Co. Ltd

• Kimura K

  Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University

• Maruyama Junichi

  Department Of Biotechnology The University Of Tokyo

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