Brewing Properties of Sake Yeast Whose EST2 Gene Encoding Isoamyl Acetate-Hydrolyzing Esterase Was Disrupted
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概要
- 論文の詳細を見る
The EST2 gene, encoding an isoamyl acetate-hydrolyzing esterase, was disrupted in a diploid strain of Saccharomyces cerevisiae UT-1(MATa/MATα ura3/ura3 trp1/trp1 EST2/EST2), which is derived from the industrial sake yeast Kyokai no. 701(strain K-701), by usins two disruption plasmids(pDest2U, est2::URA3; and pDest2T, est2::TRP1)sequentially. Genomic Southern blot analysis revealed that both loci of the EST2 gene on the chromosome of strain UT-I were disrupted. The resultant mutants were named UTUT-1 and UTUT-2(MATa/MA Tα ura3/ura3 trp1/trp1 est2::URA3/est2::TRP1). Deficiency in Est2p csterase was also confirmed by activity staining oE the gel after native-polyacrylamide gel electrophoresis of cell extracts of the two mutant strains. Small scale sake brewing was carried out using these sake yeasts and the strains they were derived from, and their brewing properties were compared. The fermentation profiles of the four strains(strains K-701, UT-1, UTUT-1, and UTUT-2)were largely similar. The components of the resulting sake were also similar except for the acetate estcr concentration, although strains UTUT-1 and UTUT-2 produced approximately 2-times more isoamyl acetate than the wild type K-701. These results strongly suggest that the EST2 gene product is likely to play a crucial role in the hydrolysis of isoamyl acetate in the sake mash. Strains UTUT-1 and UTUT-2, deficient in Est2p esterase, are suitable for sake brewing.
- 社団法人日本生物工学会の論文
- 1998-01-25
著者
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Kitamoto Katsuhiko
Department of Biotechnology, The University of Tokyo
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Inoue Y
Research Institute For Food Science Kyoto University
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Inoue Yoshiharu
Research Institute For Food S-cience Kyoto University
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Kitamoto K
Univ. Tokyo Tokyo Jpn
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Kitamoto K
National Research Institute Of Brewing
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KIMURA AKIRA
Research Institute for Food Science, Kyoto University
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Kimura A
Kyoto Univ. Kyoto
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Kimura Akira
Research Institute For Food Science Kyoto University
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Kitamoto Katsuhiko
Department Of Biotechnology University Of Tokyo
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FUKUDA KIYOSHI
Kizakura Sake Brewing Co. Ltd.
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YAMAMOTO NAGI
Kizakura Sake Brewing Co. Ltd.
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KIYOKAWA YOSHIFUMI
Kizakura Sake Brewing Co. Ltd.
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YANAGIUCHI TOSHIYASU
Kizakura Sake Brewing Co. Ltd.
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WAKAI YOSHINORI
Kizakura Sake Brewing Co. Ltd.
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Inoue Y
Kyoto Univ. Kyoto
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Kimura A
Research Institute For Food Science Kyoto University
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Kitamoto Katsuhiko
Department Of Applied Biological Chemistry And Department Of Applied Biological Engineering Graduate
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Kiyokawa Yoshifumi
Kizakura Co. Ltd.
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Wakai Yoshinori
Kizakura Co. Ltd.
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Kimura K
Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokkaido University
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Kiyokawa Yoshifumi
Kizakura Co., Ltd.
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Wakai Yoshinori
Kizakura Co., Ltd.
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Kimura Akira
Research Center for Solar Energy Chemistry, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
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