Oxidative Stress Response in Yeast: Purification and Some Properties of Oxidative Stress-inducible Glucose-6-phosphate Dehydrogenase from Hansenula mrakii
スポンサーリンク
概要
- 論文の詳細を見る
Glucose-6-phosphate dehydrogenase in a yeast, Hansenula mrakii IFO 0895 is induced when the cells are cultured in a medium containing lipid hydroperoxide. The enzyme was purified from H. mrakii to the homogeneous state on polyacrylamide gel electrophoresis. The molecular weight of the purified enzyme was estimated to be approximately 52 kDa by SDS-PAGE and 130 kDa by Sephadex G-150 column chromatography, respectively. The enzyme was specific to glucose-6-phosphate and NADP^+, and K_m values for glucose-6-phosphate and NADP^+ were 293 μM and 24.1 μM, respectively. The enzyme activity was inhibited by diethylpyrocarbonate and 2,4,6-trinitrobenzene sulfonate, and by metal ions such as Zn^<2+>,Cd^<2+>, Cu^<2+>, and Al^<3+>. tert-Butyl hydroperoxide, a kind of lipid hydroperoxide, slightly (approximately 20%) increased the enzyme activity.
- 社団法人日本農芸化学会の論文
- 1996-06-23
著者
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Inoue Y
Research Institute For Food Science Kyoto University
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Inoue Yoshiharu
Research Institute For Food S-cience Kyoto University
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Izawa Shingo
Research Institute For Food Science Kyoto University
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KIMURA AKIRA
Research Institute for Food Science, Kyoto University
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Kimura A
Kyoto Univ. Kyoto
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Kimura Akira
Research Institute For Food Science Kyoto University
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Miki Takeo
The Inst. Of Enology And Viticulture Yamanashi Univ.
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Inoue Y
Kyoto Univ. Kyoto
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Kimura A
Research Institute For Food Science Kyoto University
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MIKI Takeo
Research Institute for Food Science, Kyoto University
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TSUJIMOTO Yoshiyuki
Research Institute for Food Science, Kyoto University
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SUGIYAMA Kei-ichi
Research Institute for Food Science, Kyoto University
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MIYASE Shinji
Research Institute for Food Science, Kyoto University
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Sugiyama K
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Sakata Kanzo
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Miyase Shinji
Research Institute For Food Science Kyoto University
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Tsujimoto Yoshiyuki
Research Institute For Food Science Kyoto University
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Sakata K
Institute For Chemical Research Kyoto University
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Miki T
The Institute Of Enology And Viticulture Yamanashi University
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Kimura Akira
Research Center for Solar Energy Chemistry, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
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