Production of Recombinant Tuna Growth Hormone by a Yeast, Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
The cDNA sequence coding for tuna growth hormone (tGH) was placed under the control of the repressible acid phosphatase (PHO5) promoter of a yeast, Saccharomyces cerevisiae, in an expression plasmid, pAM82. The yeast cells transformed with the plasmid synthesized tGH only when the cDNA was attached to the vector through a synthetic oligonucleotide linker having a similar sequence to the 5'-flanking region of the PHO5 structural region. The amount of tGH produced in yeast cells accounted for more than 3% of the total cellular protein and the product was immunologically identified as tGH by Western blotting using polyclonal antibodies specific to tGH.
- 社団法人日本農芸化学会の論文
- 1989-11-23
著者
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Inoue Y
Research Institute For Food Science Kyoto University
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Inoue Yoshiharu
Research Institute For Food S-cience Kyoto University
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SATO Nobuyuki
Institute for Research and Development, Taiyo Fisheries Co., Ltd.
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KIMURA Shoji
Institute for Research and Development, Taiyo Fisheries Co., Ltd.
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NONAKA Michio
Institute for Research and Development, Taiyo Fisheries Co., Ltd.
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MURATA KOUSAKU
Research Institute for Food Science, Kyoto University
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KIMURA AKIRA
Research Institute for Food Science, Kyoto University
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Kimura Akira
Research Institute For Food Science Kyoto University
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Nonaka M
Institute For Research And Development Taiyo Fishery Co. Ltd.
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Sato N
Institute For Research And Development Taiyo Fishery Co. Ltd.
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Kimura A
Hokkaido Univ. Sapporo Jpn
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Kimura Shoji
Institute For Research And Development Taiyo Fishery Co. Ltd.
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Inoue Y
Kyoto Univ. Kyoto
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Sato Nobuyuki
Institute For Research And Development Taiyo Fishery Co. Ltd.
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Murata Kousaku
Research Institute For Food Science Kyoto Univeristy
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HAYAMI Tsuyoshi
Institute for Research and Development, Taiyo Fishery Co., Ltd.
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ICHIRYU Takayuki
Research Institute for Food Science, Kyoto University
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Kimura Shoji
Department Of Chemistry And Chemical Engineering University Of Tokyo
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Kimura Shouji
Taiyo Central R&d Institute Taiyo Fishery Co. Ltd.
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Nonaka Michio
Institute For Research And Development Taiyo Fishery Co. Ltd.
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Ichiryu Takayuki
Research Institute For Food Science Kyoto University
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Hayami Tsuyoshi
Institute For Research And Development Taiyo Fishery Co. Ltd.
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Kimura Akira
Research Center for Solar Energy Chemistry, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
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