Isolation and Properties of a Collagenase with Caseinolytic Activity from a Pseudomonas sp.
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概要
- 論文の詳細を見る
A bacterium having collagenolytic activity was isolated from soil and was identified as a Pseudomonas sp. The bacterium had two kinds of collagenolytic enzymes and one of them was purified to apparent homogeneity judging from polyacrylamide gel electrophoresis and ultracentrifugation. The purified enzyme had a molecular weight of about 45,000 and hydrolyzed insoluble collagen and synthetic oligopeptids at the site Xaa-Gly most efficiently at pH 7.4 and 45℃. Besides collagenolytic activity, the purified enzyme showed caseinolytic activity. The collagenase had a serine residue in an active site and catalyzed the hydrolysis of both collagen and casein.
- 公益社団法人日本生物工学会の論文
- 1989-12-25
著者
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Kimura Akira
Research Institute For Food Science Kyoto University
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Kimura A
Hokkaido Univ. Sapporo Jpn
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Abe S
Ehime Univ. Ehime Jpn
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Murata Kousaku
Research Institute For Food Science Kyoto Univeristy
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Kimura A
Research Institute For Food Science Kyoto University
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HISANO TOMOHIRO
Laboratory of Biotechnology, Gunze Institute
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ABE SHIRO
Laboratory of Biotechnology, Gunze Institute
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WAKASHIRO MICHIO
Laboratory of Biotechnology, Gunze Institute
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Wakashiro Michio
Department Of Biotechnology Kyoto Institute Gunze Co. Ltd.
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