ATP Regeneration with Enzymes of the Alcohol Fermentation Pathway and Kinases of Yeast and Its Computer Simulation
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概要
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Enzymes of the alcohol fermentation pathway, adenosine kinase and adenylate kinase were extracted by incubating acetone-dried yeast cells with a simple reaction medium containing glucose, and subjected to gel filtration to remove the intermediates of alcohol fermentation formed during incubation. By using the enzyme systems as catalysts and glucose as an energy source, ATP was regenerated from adenosine. A minimum concentration of fructose 1,6-bisphosphate (FBP) of 7 mM or of ATP of 10 mM was necessary to initiate the alcohol fermentation; and concentrations of nucleotides changed abruptly with reaction time. These nonlinear phenomena might be due to action of FBPase and/or ATPase as well as the complex multienzyme systems. To understand the experimentally observed phenomena, a mathematical model of the reaction system was proposed which takes into account ATP regeneration. The calculated time-dependent concentrations of glucose, FBP, adenosine and nucleotides were in agreement with experimental values qualitatively as well as quantitatively. Moreover, the nonlinear phenomena, that is, the existence of threshold concentrations of FBP and ATP below which the reaction can not proceed and the steep changes of nucleotide concentrations were also accounted for. These results indicate that the model was quite suitable for this reaction system and useful for predicting the experimental results.
- 公益社団法人日本生物工学会の論文
- 1981-08-25
著者
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Kimura Akira
Research Institute For Food Science Kyoto University
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MATSUNO RYUICHI
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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KAMIKUBO TADASHI
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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Asada Masanori
Deparment Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Nakanishi Kazuhiro
Department Of Anesthesia Nippon Medical School
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Shirai Yoshihito
Department Of Biochemical Engineering And Science Faculty Of Computer Science And Systems Engineerin
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Shirai Yoshihito
Deparment Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Matsuno Ryuichi
Deparment Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Nakanishi Kazuhiro
Deparment Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Kamikubo Tadashi
Deparment Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Kimura Akira
Research Center for Solar Energy Chemistry, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
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