NADPH-dependent Reduction of Ethyl Acetoacetate Coupled with Ethanol Oxidation in Kloeckera magna
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概要
- 論文の詳細を見る
We evaluated the catalytic ability of 29 yeast strains to reduce ethyl acetoacetate (EA) in the presence of ethanol or glucose. In 18 yeast strains, the reduction in the presence of ethanol proceeded as well as in the presence of glucose. Among them, Kloeckera magna (AKU 4704) effectively catalyzed the NADPH-dependent reduction of EA in the presence of ethanol. In this reduction, 1 mol of EA was reduced by consuming 1 mol of ethanol. We found that the NADPH regeneration system responsible for EA reduction in K. magna was coupled with the oxidation of acetaldehyde to acetic acid catalyzed by an NADP^+ -dependent aldehyde dehydrogenase.
- 社団法人日本農芸化学会の論文
- 1997-08-23
著者
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KOMETANI Tadashi
Department of Chemical and Biochemical Engineering
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Sakai Yukiko
Department Of Chemical And Biochemical Engineering Toyama National College Of Technology
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YOSHII Hidefumi
Department of Biotechnology, Tottori University
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MATSUNO RYUICHI
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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Yoshii H
Tottori Univ. Tottori Jpn
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Yoshii Hidefumi
Department Of Biochemical Engineering Toyama National College Of Technology
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MATSUNO Ryuichi
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
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Matsuno R
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Kobayashi T
Kyoto Univ. Kyoto Jpn
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URAI HISAE
Department of Chemical and Biochemical Engineering, Toyama National College of Technology
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Kometani Tadashi
Department Of Biochemical Engineering Toyama National College Of Technology
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Urai Hisae
Department Of Chemical And Biochemical Engineering Toyama National College Of Technology
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Yoshii Hidefumi
Department Of Applied Biological Science Kagawa University
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Matsuno R
Kyoto Univ. Kyoto Jpn
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Matsuno Ryuichi
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Matsuno Ryuichi
Deparment Of Food Science And Technology Faculty Of Agriculture Kyoto University
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