Kinetic Study on Δ^1-Dehydrogenation of Hydrocortisone by Gel-Entrapped Arthrobacter simplex Cells
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概要
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A kinetic study on Δ^1-dehydrogenation of hydrocortisone by Arthrobacter simplex acetone-dried cells entrapped with urethane prepolymers in 10% methanol was carried out, especially to examine the effect of gel hydrophobicity on the activity. The partition coefficient of water-insoluble hydrocortisone solubilized in 10% methanol increased with increased hydrophobicity of the gel, which indicated accumulation of the substrate, hydrocortisone, in the gel. However, the activity of the entrapped cells in Δ^1-dehydrogenation of hydrocortisone was almost independent of the hydrophobicity of the gel. The diffusion coefficient of hydrocortisone, which represented the permeability of the substrate into the gel, decreased with increased hydrophobicity of the gel. The apparent K_m value for hydrocortisone of free acetone-dried cells was larger than that of the cells entrapped in either hydrophilic or hydrophobic gels when the diffusion barrier of the gel matrix was negligible. These results suggested hydrophobic interaction between hydrocortisone and the gel matrices. Kinetic studies showed that in hydrophilic gels the site of entrapment of cells coincided with the site of localization of substrate, while in hydrophobic gels it did not. It was concluded that the activity of polyurethane gel-entrapped A. simplex cells is affected not only by the hydrophobicity of the cell-entrapping gel, which reflects the affinity of substrate for the gel, but also by such factors as the cell-entrapping site in the gel.
- 社団法人日本生物工学会の論文
- 1984-04-25
著者
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TANAKA ATSUO
Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemist
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Sonomoto Kenji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Sonomoto Kenji
Laboratory Of Industrial Biochemistry Department Of Industroal Chemistry Faculty Of Engineering Kyot
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Sonomoto Kenji
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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MATSUNO RYUICHI
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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Fukui Saburo
Laboratory Of Industrial Chemistry Depaetment Of Industrial Chemistry Faculty Of Engineering Kyoto U
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Fukui Saburo
Laboratory Of Industrial Biochemisryt Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Tanaka Atsuo
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chamistr
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Matsuno Ryuichi
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Fukui Saburo
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Tanaka Atsuo
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Matsuno Ryuichi
Deparment Of Food Science And Technology Faculty Of Agriculture Kyoto University
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