Localization and Interaction of the Biosynthetic Proteins for the Lantibiotic, Nukacin ISK-1
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概要
- 論文の詳細を見る
Nukacin ISK-1 is a type-A(II) lantibiotic produced by Staphylococcus warneri ISK-1. In this study, we characterized NukM and NukT, which are predicted to be involved in modification of prepeptide (NukA) and cleavage of leader peptide and subsequent secretion respectively. Localization analysis of NukM and NukT in the wild-type strain indicated that both proteins were located at the cytoplasm membrane. Interestingly, NukM expressed heterologously in St. carnosus TM300 was also located at the cytoplasm membrane even in the absence of NukT. Yeast two-hybrid assay showed that a complex of at least two each of NukM and NukT was associated with NukA. In vitro interaction analysis by surface plasmon resonance biosensor further suggested that membrane-located NukM interacted with NukA. These results indicate that NukM and NukT form a membrane-located multimeric protein complex and that post-translational modification of nukacin ISK-1 would occur at the cytoplasm membrane.
- 社団法人 日本農芸化学会の論文
- 2005-07-23
著者
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Aso Y
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Nakayama J
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Nakayama Jiro
Lab. Of Microbial Technol. Div. Of Microbial Sci. And Technol. Dep. Of Bioscience And Biotechnology
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Nakayama Jiro
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Nakayama Jiro
Department Of Applied Biological Chemistry Graduate School Of Agriculture And Life Sciences The Univ
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Nagao Jun-ichi
Dep. Of Functional Bioscience Section Of Infection Biology Fukuoka Dental Coll. 2-15-1 Tamura Sawara
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Nagao Jun-ichi
Department Of Functional Bioscience Section Of Infection Biology Fukuoka Dental College
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Sonomoto Kenji
Lab. Of Microbial Technol. Div. Of Microbial Sci. And Technol. Dep. Of Bioscience And Biotechnology
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Sonomoto K
(present Address)department Of Biochemical Engineering And Science Faculty Of Computer Science And S
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Sonomoto Kenji
Lab. Of Functional Food Design Dep. Of Functional Metabolic Design Bio-architecture Center Kyushu Un
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Sonomoto Kenji
Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyoto University:(
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Sonomoto Kenji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Sonomoto Kenji
Laboratory Of Microbial Technology Department Of Food Science And Technology Faculty Of Agriculture
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Sonomoto Kenji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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SASHIHARA Toshihiro
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios
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ADACHI Asaho
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios
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Sonomoto Kenji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Sonomoto Kenji
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Sonomoto Kenji
Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture
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Aso Yuji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Adachi A
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Shioya Kouki
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios
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NAGAO Jun-ichi
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios
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Shioya Kouki
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Sashihara Toshihiro
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Sonomoto Kenji
Lab. Of Functional Food Design Dep. Of Functional Metabolic Design Bio-architecture Center Kyushu Un
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Nagao Jun-ichi
Laboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
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