Effect of Chemical Modification of Tyrosine Residues on Activities of Bacterial Lipase

概要

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Lipase from Pseudomonas sp. KWI-56 was subjected to chemical modification of amino acid residues. When His and Arg residues and carboxyl groups were modified, both lipase activity and esterase activity of the lipase were remarlably decreased. Modification of tyrosine residues of the lipase with tetranitromethane resulted in a decrease in the lipase activity to hydrolyze acylglycerides and their derivatices without an appreciable loss of the esterase activity to hydrolyze synthetic esters It was found that the modification of at least one or two tyrosine residues among the 15 residues in a lipase molecule was enough to regulate the enzymatic functions described above.
1991-11-25