Growth Inhibition of Human Leukemia HL-60 Cells by an Antisense Phosphodiester Oligonucleotide Encapsulated into Fusogenic Liposomes
スポンサーリンク
概要
- 論文の詳細を見る
We report here the antisense effect of phosphodiester oligdoeoxynucleotide (D-ODN) using fusogenic liposomes (FL) as its carrier. FL has envelope proteins of the Sendai virus within its membrane and introduces its contents directly and efficiently into cytosol by means of the virus-cell fusion mechanism. Using antisense (AS) D-ODN 15-mer complementary to the c-myc proto-oncogene mRNA, including the translation initiation codon site, we analyzed the growth of HL-60 cells by [^3H]-thymidine uptake. AS-ODNs encapsulated in FL inhibited the growth by about 70% that of the control HL-60 cells at 2.48 μM. It contrast, sense and scramble D-ODNs encapsulated in FL showed no effect of the growth of HL-60 cells at the same concentration. Even at 50 μM, free form D-ODNs did not show any effect. These results suggest that FL is potentially a useful delivery vehicle for oligonucleotide-based therapeutics, and that D-ODN may be a likely candidate for oligodeoxynucleotides when an efficient delivery system is used.
- 公益社団法人日本薬学会の論文
- 2000-08-01
著者
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Sato Masao
Faculty of Pharmaceutical Science, Tokushima Bunri University
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提 康央
Faculty Of Pharmaceutical Sciences Osaka University
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真弓 忠範
Graduate School Of Pharmaceutical Sciences Kobe-gakuin Univ.
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村上 智彦
神戸学院大学 薬
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近藤 昌夫
Graduate School Of Pharmaceutical Sciences Osaka University:department Of Pharmaceutics And Biopharm
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Nakagawa S
Graduate School Of Pharmaceutical Sciences Osaka University
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Nakagawa Shinsaku
Faculty Of Pharmaceutical Sciences Osaka University
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Nakagawa Shinsaku
Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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Tsutsumi Y
Dep. Of Toxicology And Safety Sci. Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Tsujino Masaki
大阪大学 薬学研究科薬剤学
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SUZUKI Ryo
Faculty and Graduate School of Pharmaceutical Sciences, Osaka University
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TSUTSUMI Yasuo
Faculty and Graduate School of Pharmaceutical Sciences, Osaka University
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MAYUMI Tadanori
Faculty and Graduate School of Pharmaceutical Sciences, Osaka University
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近藤 昌夫
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Suzuki Ryo
Graduate School Of Pharmaceutical Sciences Nagoya City Univ.
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Sato Masao
Department Of Legal Medicine Fukushima Medical University School Of Medicine
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Sato Masao
Laboratory Of Pharmaceutical Proteomics National Institute Of Biomedical Innovation:department Of Bi
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Suzuki R
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Kawase M
Research And Development Center Kobayashi Pharmaceutical Co. Ltd.
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KONDOH Masuo
Faculty of Pharmaceutical Sciences, Tokushima Bunri University
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MATSUYAMA Takahiro
Tanabe Seiyaku Co., Ltd. Pharmaceutics Research Laboratory
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MIZUGUCHI Hiroyuki
National Institute of Health Sciences
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NAKANISHI Tsuyoshi
Faculty of Pharmaceutical Sciences, Osaka University
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NAKANISHI Mahito
Research Institute for Microbial Diseases
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Mayumi Tadanori
Faculty Of Pharmacy Osaka University
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Mayumi Tadanori
Dep. Of Biopharmaceutics Grad. Sch. Of Pharm. Sci. Osaka Univ.
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Suzuki R
Electrotechnical Lab. Ibaraki
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堤 康央
医薬基盤研究所:大阪大学大学院薬学研究科:大阪大学臨床医工学融合研究教育センター
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Suzuki R
Graduate School Of Engineering Tokyo Metropolitan University
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Sato M
Department Of Legal Medicine Fukushima Medical University School Of Medicine
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Mizuguchi Hiroyuki
Lab. Of Gene Transfer And Regulation National Inst. Of Biomedical Innovation
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Kondoh Masuo
Faculty Of Pharmaceutical Sciences Tokushima Bunri University
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Mayumi Tadanori
Faculty Of Pharmaceutical Sciences Kobe-gakuin University:faculty Of Pharmaceutical Sciences Osaka U
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Tsutsumi Yasuo
Dep. Of Biopharmaceutics Grad. Sch. Of Pharm. Sci. Osaka Univ.
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Nakanishi Tsuyoshi
Faculty Of Pharmaceutical Sciences Osaka University
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Matsuyama Takahiro
Tanabe Seiyaku Co. Ltd. Pharmaceutics Research Laboratory
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Mizuguchi H
Laboratory Of Gene Transfer And Regulation National Institute Of Biomedical Innovation:graduate Scho
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Sato Masao
Faculty Of Pharmaceutical Science Tokushima Bunri University
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Nakagawa Shinsaku
Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University
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