Pigments from Uroleucon nigrotuberculatum Induce Apoptosis in HL60 Human Leukemia Cells, Implicating Intracellular Oxidative Stress and Activation of Caspases(Molecular and Cell Biology)

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Two pigmented compounds from Uroleucon nigrotuberculatum, rhododactynaphin-jc-1 (H427) and rhododactynaphin-jc-2 (H373), significantly diminished the cell viability of HL60 cells with IC_<50> of 10μM and 30μM, respectively, in an 18 h-dye uptake assay. Both H427 and H373 augmented the levels of intracellular reactive oxygen species (ROS) and induced apoptosis as demonstrated by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling analysis. ROS augmentation by both H427 and H373 was inhibited by N-acetylcysteine (NAC) and α-tocopherol. The apoptosis induced by H427 was inhibited efficiently with NAC and caspase-8 inhibitor but less efficiently with α-tocopherol and caspase-9 inhibitor. These findings suggested that these pigments have pro-apoptotic activities via oxidative stress.
公益社団法人日本薬学会の論文
2006-12-01