Activation of Caspase-3 in HL-60 Cells Treated with Pyrithione and Zinc(Toxicology)
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概要
- 論文の詳細を見る
The transition metal zinc (Zn) is an endogenous regulator of apoptosis. The ability of Zn to modulate apoptosis is believed to be mediated by the regulation of caspase activity. Previously, we reported that an acute influx of labile Zn induced apoptosis via activation of caspase in human leukemia HL-60 cells treated with a Zn ionophore (Py, pyrithione) and Zn at 1 and 25μM, respectively. In the present study, we investigated the involvement of caspase-3 in Py (1μM)/Zn (25μM)-induced apoptosis in HL-60 cells. Pro-caspase-3 is an inactive form of caspase-3. The processing of pro-caspase-3, a sign of caspase-3 activation, occurred 6h after treatment with Py/Zn. Proteolysis of poly (ADP-ribose) polymerase (PARP), a substrate of caspase-3, was also observed 6h after treatment with Py/Zn. We also confirmed the elevation of caspase-3 activity as an index of the cleavage of amino acid sequences recognized by activated caspase-3. An inhibitor of caspase-3 attenuated the appearance of the DNA ladder. Taken together, these results indicate that the activation of caspase-3 is partly responsible for the induction of apoptosis in Py/Zn-treated HL-60 cells.
- 公益社団法人日本薬学会の論文
- 2005-04-01
著者
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Sato Masao
Faculty of Pharmaceutical Science, Tokushima Bunri University
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近藤 昌夫
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Watanabe Yoshiteru
Department of Pharmaceutics and Biopharmaceutics, Showa Pharmaceutical University
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Sato Masao
徳島文理大学 薬学部
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KONDOH Masuo
Department of Biopharmaceutics and Pharmaceutics, Showa Pharmaceutical University
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HIGASHIMOTO Minoru
Faculty of Pharmaceutical Sciences, Tokushima Bunri University
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TAKIGUCHI Masufumi
Faculty of Pharmaceutical Sciences, Tokushima Bunri University
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Watanabe Yoshiteru
Department Of Pharmaceutics And Biopharmaceutics Showa Pharmaceutical University
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Kondoh Masuo
Faculty Of Pharmaceutical Sciences Tokushima Bunri University
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TASAKI Emi
Faculty of Pharmaceutical Sciences, Tokushima Bunri University
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Higashimoto Minoru
Faculty Of Pharmaceutical Sciences Tokushima Bunri University:faculty Of Food And Nutrition Suzugami
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Tasaki Emi
Faculty Of Pharmaceutical Sciences Tokushima Bunri University
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Takiguchi Masufumi
Faculty Of Pharmaceutical Sciences Tokushima Bunri University:faculty Of Pharmaceutical Sciences Hir
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Sato Masao
Faculty Of Pharmaceutical Science Tokushima Bunri University
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