Response of Human Epithelial Cells to Culture Surfaces with Varied Roughnesses Prepared by Immobilizing Dendrimers with/without D-Glucose Display(CELL AND TISSUE ENGINEERING)

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To investigate the response of human epithelial cells to substrates with nanoscale modifications, dendrimerimmobilized surfaces were prepared with or without D-glucose displayed as a terminal ligand, giving topographic structures with mean roughnesses (R_a) of 1.8-11.0nm. With an increase in the R_a value up to 4.0 nm, the epithelial cells cultured on naked dendrimer surface without D-glucose display were somewhat stretched in their morphology compared with those on a nonmodified plain surface. However, for the R_a values higher than 4.0nm, such cell stretching was inhibited, resulting in the predominant existence of round-shaped cells. The change in cell morphology was appreciable on the surfaces with D-glucose-displayed dendrimers. When the R_a value increased up to 4.5nm on these surfaces, in particular, the enhancement of cell stretching was recognized, and fluorescence microscopic observation supported the hypothesis that the glucose-transporter-mediated adhesion of cells to the surface encouraged the development of filopodia and stress fibers, thereby improving focal contact with the surface. Our results suggest that the combination of displaying D-glucose and modulating roughness can promote cytoskeletal formation accompanied by marked cell elongation on culture surfaces.
社団法人日本生物工学会の論文
2007-02-25

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