Image Cytometry for Analyzing Regional Distribution of Cells Inside Human Neurospheres(CELL AND TISSUE ENGINEERING)
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概要
- 論文の詳細を見る
An image cytometry algorithm was developed to identify and quantify immunologically labeled cells in cultured human neurospheres. Image cytometry revealed new findings in terms of the localization of specific types of neural cells and the regional fluctuation of cell density in neurospheres. The distributions of total cell density exhibited similar profiles along the radial distance from the periphery of a neurosphere, irrespective of neurosphere sizes examined; approximately 1.0×10^6 cell/mm^3 in the vicinity of the periphery and 6.0×10^5 cell/mm^3 in an inner region of 50-180 μm. In addition, BrdU-positive(proliferating) cells were present at ratios of 53-64% acrossthe entire region of the neurospheres, whereas BrdU-negative and β III tubulin-positive cells(post-mitotic neurons) were minorities in the neurospheres.
- 社団法人日本生物工学会の論文
- 2007-04-25
著者
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Mori Hideki
Department of Biological Science, Graduate School of Science, Osaka Prefecture University
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Taya Masahito
Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of En
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紀ノ岡 正博
阪大院・工・生命先端
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MORI Hideki
Institute of Laboratory Animals, Gifu University School of Medicine
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田谷 正仁
大阪大学大学院基礎工学研究科 化学系専攻
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Mori Hideki
Dep. Of Biological Sci. Graduate School Of Sci. Osaka Prefecture Univ. 1-2 Gakuen-cho Naka-ku Sakai
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Kanemura Yonehiro
Institute for Clinical Research, Osaka National Hospital, National Hospital Organization
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Kino-oka Masahiro
Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University
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Ninomiya Kazuaki
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Techn
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Yamasaki Mami
Institute for Clinical Research, Osaka National Hospital, National Hospital Organization
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Yamasaki Mami
Department Of Neurosurgery Osaka National Hospital National Hospital Organization:department Of Mole
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Kanemura Yonehiro
Stem Biomethod Corporation
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Kanemura Yonehiro
Institute For Clinical Research
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紀ノ岡 正博
Dep. Of Biotechnology Graduate School Of Engineering Osaka Univ. 2-1 Yamadaoka Suita Osaka 565-0871
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Kino-oka Masahiro
Grad. Sch. Eng. Sci. Osaka Univ.
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Taya Masahito
Div. Of Chemical Engineering Graduate School Of Engineering Sci. Osaka Univ. 1-3 Machikaneyama-cho T
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Taya M
Nagoya Univ. Nagoya Jpn
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Taya Masahito
Division Of Chemical Engineering Department Of Materials Engineering Science Graduate School Of Engi
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Yamasaki Mami
Institute For Clinical Research
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Ninomiya K
Research Institute For Cell Engineering National Institute Of Advanced Industrial Science And Techno
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Kino-oka Masahiro
Division Of Chemical Engineering Department Of Biotechnology Graduate School Of Engineering Osaka Un
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Kanemura Yonehiro
Institute For Clinical Research Osaka National Hospital National Hospital Organization:research Inst
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Mori Hideki
Institute Of Geosciences Shizuoka University
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Mori Hideki
Division Of Chemical Engineering Graduate School Of Engineering Science Osaka University:institute F
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Mori Hideki
Inst. For Clinical Res. Osaka National Hospital National Hospital Organization
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Mori Hideki
Institute of Diabetes Research Center, Takanoko Hospital
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