In vitro osteogenic differentiation of HOS cells on two types of collagen gels(CELL AND TISSUE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
HOS cell is a model strain of human osteoblasts derived from human osteosarcoma. We cultured the HOS cells on both the conventional collagen gel (neutral gel), and the gamma-crosslinked collagen gel without collagen fibrils (acidic gel). The shape of HOS cells on the neutral gel was similar to that on the culture dish. However, HOS cells on acidic gel had an elongated shape and attached each other to form a mesh-like pattern. The cells attached to the surface of both gels but scarcely penetrated their depths. We measured the biochemical markers for osteogenic differentiation in the HOS cells cultured on both the neutral gel and the acidic gel. The expressions of alkaline phosphatase and osteocalcin were detected in the HOS cells on both types of collagen gel. Deposition of the calcium also occurred on both gels although it was higher in the neutral gel than the acidic one. These results indicate the importance of collagen for the differentiation of HOS cells, but it is not dependent on the molecular structure (fibril formation) of collagen.
著者
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Mori Hideki
Department of Biological Science, Graduate School of Science, Osaka Prefecture University
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Hara Masayuki
Department of Biological Science, Graduate School of Science, Osaka Prefecture University
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Takitoh Takako
Department of Biological Science, Graduate School of Science, Osaka Prefecture University
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Kato Yoichi
Research Institute for Cell Engineering, AIST
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Nakasu Asako
Research Institute for Cell Engineering, AIST
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Tadokoro Mika
Research Institute for Cell Engineering, AIST
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Bessho Masahiko
Department of Biological Science, Graduate School of Science, Osaka Prefecture University
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Hirose Motohiro
Research Institute for Cell Engineering, AIST
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Ohgushi Hajime
Research Institute for Cell Engineering, AIST
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Nakasu Asako
Research Institute For Cell Engineering Aist
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Tadokoro Mika
Research Institute For Cell Engineering Aist
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Ohgushi Hajime
Research Institute For Cell Engineering Aist
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Ohgushi Hajime
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Takitoh Takako
Department Of Biological Science Graduate School Of Science Osaka Prefecture University
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Hirose Motohiro
Research Institute For Cell Engineering Aist
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Hirose Motohiro
Research Institute For Cell Engineering (rice) National Institute Of Advanced Industrial Science And
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Bessho Masahiko
Department Of Biological Science Graduate School Of Science Osaka Prefecture University
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Mori Hideki
Department Of Anatomy Okayama University Medical School
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Hara Masayuki
Department Of Biological Science Graduate School Of Science Osaka Prefecture University
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Kato Yoichi
Research Institute For Cell Engineering Aist
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Hara Masayuki
Department Of Applied Bioscience Institute For Advanced Science And Technology Osaka Prefecture Univ
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Hara Masayuki
Department Of Anatomy St. Marianna University School Of Medicine
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