Melatonin Binding Sites in the Goldfish Retina

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概要

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• Melatonin binding sites in the goldfish retina were characterized by radioreceptor assay using 2-[^<125>] iodomelatonin as the radioligand. The specific binding to goldfish retinal membranes is rapid, stable, saturable and reversible. Saturation studies demonstrated that 2-[^<125>] iodomelatonin binds to a single class of sites with an affinity constant (K_d) of 61.9±5.7 pM, a total binding capacity (B_<max>) of 6.52±0.79 fmol/mg protein and Hill coefficient (n_H) of 1.07±0.03 (mean±SEM, n=6). Competition experiments with various indoles and neurotransmitters revealed the following order of affinities : 2-iodomelatonin>melatonin>6-hydroxymelatonin>5-methoxytryptamine=N-acetylserotonin>5-methoxytryptophol. The other indoles and neurotransmitters tested were much less effective. The order resembles with those reported for the goldfish brain and the ML-1 subtype melatonin receptors in vertebrates. Co-incubation of retinal membranes with a non-hydrolyzable GTP analog, guanosine 5'-O-(3-thiotriphosphate), significantly reduced the specific binding. These results suggest that in the goldfish, ocular melatonin plays neuromodulatory roles inb the retina via G protein-coupled melatonin receptors with picomolar affinity.

• 社団法人日本動物学会の論文

著者

• Hara Masayuki

  Department of Biological Science, Graduate School of Science, Osaka Prefecture University

• IIGO Masayuki

  Department of Applied Biochemical Science, Faculty of Agriculture, Utsunomiya University

• Iigo M

  Department Of Applied Biochemistry Faculty Of Agriculture Utsunomiya University

• Hara M

  Central Laboratory For Biomedical Research And Education Yamaguchi University School Of Medicine

• OHTANI-KANEKO RITSUKO

  Department of Anatomy, St. Marianna University School of Medicine

• HIRATA KAZUAKI

  Department of Anatomy, St. Marianna University School of Medicine

• Aida Katsumi

  Laboratory of Aquatic Animal Physiology, Graduate School of Agricultural and Life Sciences, The Univ

• Ichikawa M

  Mie Univ. Mie Jpn

• Iigo Masayuki

  Department Of Applied Biochemistry Faculty Of Agriculture Utsunomiya University

• Iigo Masayuki

  Department Of Anatomy St. Marianna University School Of Medicine

• Ohtani-kaneko Ritsuko

  Department Of Anatomy St. Marianna University School Of Medicine

• Ohtani-kaneko Ritsuko

  聖マリアンナ医科大学 解剖学

• Ohtani-kaneko Ritsuko

  Departments Of Anatomy And Surgery St.marianna University School Of Medicine

• Aida Katsumi

  Department Of Aquatic Bioscience Graduate School Of Agricultural And Sciences The University Of Toky

• Hara Masayuki

  Central Laboratory For Biomedical Research And Education Yamaguchi University School Of Medicine

• Hirata K

  Department Of Anatomy St. Marianna University School Of Medicine

• Hirata Kazuaki

  Department Of Anatomy St. Marianna University School Of Medicine

• Aida Katsumi

  Laboratory Of Aquatic Animal Physiology Graduate School Of Agricultural And Life Sciences The Univer

• Hara Masayuki

  Department Of Biological Science Graduate School Of Science Osaka Prefecture University

• Kezuka H

  Laboratory Of Aquatic Animal Physiology Department Of Aquatic Bioscience Graduate School Of Agricult

• Ichikawa M

  Department Of Applied Biochemistry Utsunomiya University

• Ohtani‐kaneko R

  St. Marianna Univ. School Of Medicine Kanagawa Jpn

• Hara Masayuki

  Department Of Applied Bioscience Institute For Advanced Science And Technology Osaka Prefecture Univ

• Aida K

  Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of

• Hara Masayuki

  Department Of Anatomy St. Marianna University School Of Medicine

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