A Splicing Variant for the Prepro-Mammalian Gonadotropin-Releasing Hormone (Prepro-mGnRH) mRNA is Present in the Brain and Various Peripheral Tissues of the Japanese Eel(Endocrinology)
スポンサーリンク
概要
- 論文の詳細を見る
We have previously demonstrated that the Japanese eel, Anguilla japonica, has two distinct mRNAs for prepro-mammalian gonadotropin-releasing hormone (prepro-mGnRH) and prepro-chicken GnRH-II (prepro-cGnRH-II). In the present study, we found a splicing variant for the prepro-mGnRH mRNA, but not for the prepro-cGnRH-II mRNA in the eel. Genomic Southern blot analysis revealed that each of the eel prepro-GnRHs is encoded by a single gene. Isolation and sequencing of the two prepro-GnRH genes revealed that both of them contain four exons (denoted 1, 2, 3 and 4) separated by three introns (denoted A, B and C). Staining with ethidium bromide and Southern blotting following reverse transcription-polymerase chain reaction and electrophoresis revealed that in addition to a mature prepro-mGnRH mRNA, its splicing variant with retention of intron A is present in all tissues tested including the brain, pituitary, eye, olfactory epithelium, ovary, testis, liver, kidney, spleen, heart, gill, intestine, pancreas, muscle, skin, fin and peripheral blood leukocyte. This result differed to that of mammals, the splicing variant of which is present only in the peripheral tissues, but not in the brain. In contrast, only a mature mRNA for the prepro-cGnRH-II was detected in the middle part of dissected brain, pituitary, olfactory epithelium, ovary and testis, indicating that the two prepro-GnRH genes have different splicing processes. These findings suggested that the splicing pattern which produces the intron A retention variant dates from a common ancestor to teleosts and mammals, and is conserved during evolution. Therefore the splicing may be one of the mechanisms regulating prepro-mGnRH gene expression.
- 社団法人日本動物学会の論文
- 1999-08-15
著者
-
Aida Katsumi
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University
-
Aida K
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences University Of Tok
-
Okubo K
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
-
Okubo Kataaki
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
-
Aida Katsumi
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
-
Aida Katsumi
Department Of Aquatic Bioscience Graduate School Of Agricultural And Sciences The University Of Toky
-
Okuzawa K
National Research Institute Of Aquaculture
-
Okuzawa Koichi
National Research Institute Of Aquaculture Fisheries Research Agency
-
SUETAKE Hiroaki
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University
-
Asahina K
Univ. Utrecht Utrecht Nld
-
Suetake Hiroaki
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
-
Aida K
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
関連論文
- The dynamics of vitellogenin gene expression differs between intact and eyestalk ablated kuruma prawn Penaeus (Marsupenaeus) japonicus
- Isolation and cDNA Cloning of Ovarian Cortical Rod Protein in Kuruma Prawn Marsupenaeus japonicus(Crustacea: Decapoda: Penaeidae)(Reproduction Biology)
- Influence of estradiol-17β, testosterone, and 11-ketotestosterone on testicular development, serum steroid hormone, and gonadotropin secretion in male red sea bream Pagrus major
- Effects of 11-ketotestosterone and gonadotropin-releasing hormone on follicle-stimulating hormone and luteinizing hormone gene expression in castrated and sham-operated male red seabream Pagrus major
- THE ROLE OF HIGH WATER TEMPERATURE IN THE TERMINATION OF SPAWNING OF RED SEABREAM(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- BACULOVIRUS-MEDIATED PRODUCTION OF THE RED SEABREAM FSH AND LH IN SILKWORM LARVAE(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- CLONING AND CHARACTERIZAION OF A GUINEA PIG GnRH RECEPTOR(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- PHYSICAL AND FUNCTIONAL LINKAGES BETWEEN GONADOTROPIN-RELEASING HORMONE AND TYPE IV TYROSINE PHOSPHATASE GENES(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Ontogenic Development of Three GnRH Systems in the Brain of a Pleuronectiform Fish, Barfin Flounder(Endocrinology)
- THREE GnRH SYSTEMS IN THE BRAIN OF BARFIN FLOUNDER(Endocrinology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Effects of Melatonin Feeding on Smoltification in Masu Salmon(Oncorhynchus masou)(Endocrinology)
- Effects of Macromolecule Synthesis Inhibitors on Light-Induced Phase Shift of the Circadian Rhythm in Melatonin Release from the Cultured Pineal Organ of a Teleost, Ayu (Plecoglossus altivelis)(Endocrinology)
- Variations in Plasma Melatonin Levels of the Rainbow Trout (Oncorhynchus mykiss) under Various Light and Temperature Conditions(Endocrinology)
- Retina-Type Rhodopsin Gene Expressed in the Brain of a Teleost, Ayu (Plecoglossus altivelis)(Reproductive Biology)
- Molecular Cloning and Characterization of a cDNA Encoding the Retinal Arylalkylamine N-Acetyltransferase of the Rainbow Trout, Oncorhynchus mykiss
- Molecular cloning of a cDNA that encodes arylalkylamine N-acetyltransferase from the rainbow trout, Oncorhynchus mykiss
- Effects of Photoperiod, Pinealectomy and Ophthalmectomy on Circulating Melatonin Rhythms in the Goldfish, Carassius auratus(Endocrinology)
- CLONING OF VITELLOGENIN cDNA IN THE COONSTRIPED SHRIMP, PANDALUS HYPSINOTUS
- Molecular Characterization of a cDNA Encoding Vitellogenin and Its Expression in the Hepatopancreas and Ovary during Vitellogenesis in the Kuruma Prawn, Penaeus japonicus(Reproductive Biology)
- Accumulation of Ovarian Ecdysteroids in Synchronization with Gonadal Development in the Giant Freshwater Prawn, Macrobrachium rosenbergii(Endocrinology)
- THREE DISTINCT GONADOTROPIN-RELEASING HORMONE RECEPTORS IN FUGU TAKIFUGU RUBRIPES
- Differential expression of two gonadotropin (GTH) β subunit genes during ovarian maturation induced by repeated injection of salmon GTH in the Japanese eel Anguilla japonica
- A Splicing Variant for the Prepro-Mammalian Gonadotropin-Releasing Hormone (Prepro-mGnRH) mRNA is Present in the Brain and Various Peripheral Tissues of the Japanese Eel(Endocrinology)
- EXPRESSION PATTERN OF TWO GONADOTROPIN - RELEASING HORMONE GENES IN VARIOUS TISSUES OF THE JAPANESE EEL
- Expression of Two Gonadotropin-Releasing Hormone (GnRH) Precursor Genes in Various Tissues of the Japanese Eel and Evolution of GnRH(Endocrinology)
- STRUCTURE AND EXPRESSION OF TWO FORMS OF GONADOTROPIN-RELEASING HORMONE IN THE JAPANESE EEL
- Localization and Developmental Expression of mRNA for Cortical Rod Protein in Kuruma Prawn Marsupenaeus japonicus(Reproductive Biology)
- Plasma Steroid Hormone Profiles in HCG-Injected Male Walking Catfish Clarias batrachus
- Plasma Steroid Hormone Profiles during HCG Induced Ovulation in Female Walking Catfish Clarias batrachus(Endocrinology)
- Biosynthesis of 17α,20α-dihydroxy-4-pregnen-3-one from 17α-hydroxyprogesterone by Goldfish (Carassius auratus) Spermatozoa
- THREE GnRH SYSTEMS IN THE BRAIN OF PLEURONECTIFORM FISH, BARFIN FLOUNDER, VERASPER MOSERI
- Low GnRH Levels in the Brain and the Pituitary in Triploid Female Sockeye Salmon
- Changes in Salmon GnRH and Chicken GnRH-II Contents in the Brain and Pituitary, and GTH Contents in the Pituitary in Female Masu Salmon, Oncorhynchus masou, from Hatching through Ovulation(Endocrinology)
- Characterization and Molecular Cloning of Cellobiose Dehydrogenase from the Brown-Rot Fungus Coniophora puteana(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Production and Characterization of Recombinant Phanerochaete chrysosporium Cellobiose Dehydrogenase in the Methylotrophic Yeast Pichia pastoris(Biochemistry & Molecular Biology)
- Expression of a Recombinant Molt-Inhibiting Hormone of the Kuruma Prawn Penaeus japonicus in Escherichia coli
- BACTERIAL EXPRESSION AND IN VITRO ACTIVITY OF RECOMBINANT MOLT - INHIBTING HORMONE (r - MIH) OF THE KURAMA PRAWN Penaeus japonicus
- Hemolymph Profiles of Juvenoid Substances in the Giant Freshwater Prawn Macrobrachium rosenbergii in Relation to Reproduction and Molting
- The Presence of 20-Hydroxyecdysonoic Acid and Ecdysonoic Acid in Eggs of the Giant Freshwater Prawn Macrobrachium rosenbergii
- Distribution and Function of Gonadotropin-Releasing Hormone (GnRH) in the Teleost Brain
- ROLES OF GONADOTROPIN-RELEASING HORMONE IN TESTICULAR MATURATION OF BARFIN FLOUNDER, VERASPER MOSERI
- Incomplete Development of the Brain-Pituitary-Gonadal Axis May Underlie the Delay in the Initiation of Precocious Maturation in Male Sockeye Salmon
- Molecular Cloning of cDNAs Encoding Two Pigment-Dispersing Hormones and Two Corresponding Genes from the Kuruma Prawn (Penaeus japonicus)
- Identification of Two Distinct Molt-Inhibiting Hormone-Related Peptides from the Giant Tiger Prawn Penaeus monodon
- Molecular Cloning of a Molt-Inhibiting Hormone cDNA from the Kuruma Prawn Penaeus japonicus
- IMMUNOHISTOCHEMICAL IDENTIFICATION OF HYPERGLYCEMIC HORMONE -PRODUCING CELLS AND MOLT- INHIBITING HORMONE -PRODUCING CELLS IN THE EYESTALK OF THE KURUMA PRAWN, PENAEUS JAPONICUS
- A Novel Neuropeptide with Molt-Inhibiting Activity from the Sinus Gland of the Crayfish, Procambarus clarkii
- Isolation and Amino Acid Sequence of a Molt-inhibiting Hormone from the American Crayfish, Procambarus clarkii
- IDENTIFICATION OF A PEPTIDE WITH MOLT-INHIBITING ACTIVITY FROM THE SINUS GLAND OF THE CRAYFISH, PROCAMBARUS CLARKII
- Elevation of the activities of β-N-acetylhexosaminidase isozymes by 20-hydroxyecdysone administration in the kuruma prawn
- MOLECULAR CLONING OF A cDNA ENCODING A MOLT-INHIBITING HORMONE FROM THE KURUMA PRAWN Penaeus japonicus
- Effects of sex steroids on the mRNA levels of gonadotropin subunits in juvenile and ovariectomized goldfish Carassius auratus
- Nucleotide Sequence of Gonadotropin IIβ Subunit Gene in Goldfish
- REPRODUCTIVE RHYTHM AND GONADOTROPIN GENE EXPRESSION IN GOLDFISH
- Effect of Sex Steroids on the mRNA Levels of Gonadotropin I and II Subunits in the Goldfish Carassius auratus
- Effects of Water Temperature and Food Limitation on Pituitary Gonadotropin and Thyrotropin Subunit mRNA Levels in the Female Goldfish Carassius auratus
- SEASONAL CHANGES IN GENE EXPRESSION OF GONADOTROPIN AND THYROTROPIN IN GOLDFISH
- EFFECTS OF WATER TEMPERATURE, STARVATION AND SEX STEROIDS ON THE EXPRESSION OF GONADOTROPIN AND THYROTROPIN SUBUNIT GENES IN GOLDFISH
- Effects of temperature on vitellogenesis in Japanese eel Anguilla japonica
- Effects of Olfactory Tract Section on the Immunohistochemical Distribution of Brain GnRH Fibers in the Female Goldfish, Carassius auratus(Endocrinology)
- Time-course response of plasma testosterone and estradiol-17β to salmon gonadotropin treatment in the Japanese eel during ovarian development
- Adequate interval of sGTH administration to induce vitellogenesis, and induction of ovulation by simultaneous administration of sGTH and 17α-hydroxyprogesterone in the Japanese eel
- Hemolymph vitellogenin levels during ovarian development in the kuruma prawn Penaeus japonicus
- Purification and characterization of vitellin from the ovary of kuruma prawn, Penaeus japonicus
- Two salmon gonadotropin-releasing hormone genes and their differential expressions in the goldfish Carassius auratus
- Chitinolytic Enzyme Activities in the Hepatopancresas, Tail Fan and Hemolymph of Kuruma Prawn Penaeus japonicus during the Molt Cycle
- Effect of reward level on the number of food demands in yellowtail Seriola quinqueradiata
- Nocturnal/diurnal demand-feeding pattern of yellowtail Seriola quinqueradiata under different keeping conditions
- Use of an Emulsion Prepared with Lipophilized Gelatin for the Induction of Ovarian Maturation in the Japanese Eel Anguilla japonica
- USE OF AN EMULSION PREPARED WITH LIPOPHILIZED GELATIN FOR INDUCTION OF GONADAL MATURATION IN THE JAPANESE EEL, Anguilla japonica
- Effects of bilateral eyestalk ablation on molting and ovarian development in the giant freshwater prawn, Macrobrachium rosenbergii
- Inhibitory Effects of Testosterone on Downstream Migratory Behavior in Masu Salmon, Oncorhynchus masou(Behavior Biology)
- Growth of Wild Honmasu Salmon Parr in a Tributary of Lake Chuzenji
- THE INVOLVEMENT OF TESTOSTERONE IN DOWNSTREAM AND UPSTREAM BEHAVIOR OF MASU SALMON
- TWO DIFFERING SALMON GONADOTROPIN-RELEASING HORMONE PRECURSOR mRNAs EXPRESSED IN THE BRAIN OF SOCKEYE SALMON
- EFFECTS OF TESTOSTERONE ON DOWNSTREAM AND UPSTREAM BEHAVIOR OF MASU SALMON
- INHIBITORY EFFECT OF TESTOSTERONE ON THE DOWNSTREAM BEHAVIOR OF MASU SALMON SMOLTS
- ACTIVATION OF THE SYNTHESIS OF SALMON GONADOTROPIN-RELEASING HORMONE PRIOR TO DIFFERENTIATION OF PRECOCIOUS MALES IN MASU SALMON
- SEASONAL CHANGES IN LEVELS OF sGnRH AND cGnRH-II IN DISCRETE AREAS OF THE BRAIN OF THE FEMALE GOLDFISH, Carassius auratus
- MOLECULAR CLONING OF A cDNA THAT ENCODES THE PRECURSOR TO A SALMON-TYPE GONADOTROPIN-RELEASING HORMONE IN THE GOLDFISH
- Changes in Brain GnRH after Olfactory Tract Section in Female Goldfish
- Molecular cloning of cDNA encoding a 20S proteasome 2 subunit from goldfish (Carassius auratus) and its expression analysis
- Gonadotoropin Secretion in the Reproductive Cycles in Teleosts
- Vitellogenin Production Induced by Eyestalk Ablation in Juvenile Giant Freshwater Prawn Macrobrachium rosenbergii and Trial Methyl Farnesoate Administration
- Low-salinity tolerance of juvenile fugu Takifugu rubripes
- Decreased transport of orally administered protein into the blood circulation of developing juveniles of Japanese eel Anguilla japonica
- Oral immunization specifically inhibits intestinal protein uptake in the common carp Cyprinus carpio L.
- Effects of Steroids on the Antibody Producing Activity of Lymphocytes in Rainbow Trout
- Changes in Immunoglobulin Producing Cells in Response to Gonadal Maturation in Rainbow Trout
- Humoral Immune Response Against Orally Administered Human γ Globulin in the Carp
- The genes encoding the two types of gonadotropin α subunits of the masu salmon (Oncorhyncus masou)
- ACTIONS OF A GONADOTROPIN-RELEASING HORMONE ANTAGONIST ON GONADOTROPIC HORMONE RELEASE IN RAINBOW TROUT, Oncorhynchus mykiss
- DOPAMINERGIC REGULATION OF THE RELEASE OF GONADOTROPIN FROM DISPERSED PITUITARY CELLS OF TESTOSTERONE-TREATED IMMATURE RAINBOW TROUT, Oncorhynchus mykiss
- Serum Cortisol Levels in Captive Killer Whale and Bottlenose Dolphin
- Follicle-stimulating hormone β, luteinizing hormone β and glycoprotein hormone α subunit mRNA levels in artificially maturing Japanese eel Anguilla japonica and naturally maturing New Zealand longfinned eel Anguilla dieffenbachii
- Oogenesis and Changes in the Levels of Reproductive Hormones in Triploid Female Rainbow Trout
- The first description of the double-bellied condition of the digastric muscle in the finless porpoise Neophocaena phocaenoides and Dall's porpoise Phocoenoides dalli
- Immunohistochemical identification of hyperglycemic hormone- and molt-inhibiting hormone-producing cells in the eyestalk of the kuruma prawn, Penaeus japonicus
- Changes in the levels of mRNA coding for gonadotropin Iβ and IIβ subunits during vitellogenesis in the common Japanese conger Conger myriaster
- Involvement of sex steroids, luteinizing hormone and thyroid hormones in upstream and downstream swimming behavior of land-locked sockeye salmon Oncorhynchus nerka