Biosynthesis of 17α,20α-dihydroxy-4-pregnen-3-one from 17α-hydroxyprogesterone by Goldfish (Carassius auratus) Spermatozoa
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概要
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Goldfish (Carassius auratus) spermatozoa were incubated with 4-^<14>C-labeled 17α-hydroxyprogesterone at 22°C for 60 min. After extraction of the steroids from the incubation medium, they were separated by thin-layer chromatography (TLC). Radioactive steroids were localized on the TLC-plates by auto-radiography. The major metabolite showed an Rf value lower than that of 17α, 20β-dihydroxy-4-pregnen-3-one (17α, 20β-P) but co-migrated with 17α, 20α-P, the isomer of 17α, 20β-P. The TLC behaviour of the metabolite was identical with authentic 17α, 20α-P after both acetylation and oxidation. The identity of the metabolite with 17α, 20α-P was finally confirmed by repeated crystallization to constant specific activity. These results indicate that goldfish sperm cells show 20α-hydroxysteroid dehydrogenase activity.
- 社団法人日本動物学会の論文
- 1993-04-15
著者
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Aida Katsumi
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University
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Aida Katsumi
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
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Aida Katsumi
Department Of Fisheries Faculty Of Agriculture The University Of Tokyo
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Asahina Kiyoshi
College Of Agriculture And Veterinary Medicine Nihon University
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Higashi Teizo
College of Agriculture and Veterinary Medicine, Nihon University
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Higashi Teizo
College Of Agriculture And Veterinary Medicine Nihon University
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