Expression of a Recombinant Molt-Inhibiting Hormone of the Kuruma Prawn Penaeus japonicus in Escherichia coli
スポンサーリンク
概要
- 論文の詳細を見る
The crustacean molt-inhibiting hormone (MIH) suppresses ecdysteroid synthesis by the Y-organ. The MIH of the kuruma prawn Penaeus japonicus has recently been isolated and its cDNA cloned. In this study, we expressed the MIH in Escherichia coli to obtain a large quantity of this hormone with biological activity. The MIH cDNA was processed and ligated into an expression plasmid. E. coli was transformed with this plasmid, and then the recombinant MIH (r-MIH) was expressed. The r-MIH was put through the refolding reaction and was purified by reversephase HPLC. N-terminal amino acid sequence and time-of-flight mass spectral analyses supported the idea that the r-MIH had the entire sequence. By in vitro bioassay using the Y-organ of the crayfish, the r-MIH was found to be comparable to natural MIH in inhibiting ecdysteroid synthesis.
- 社団法人日本農芸化学会の論文
- 1999-09-23
著者
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Nishino Takeshi
Department Of Biochemistry And Molecular Biology Nippon Medical School
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Watanabe Toshiki
Ocean research Institute University of Tokyo
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濱本 和敏
Laboratory Of Fish Pathology School Of Fisheries Sciences Kitasato University
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Aida Katsumi
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University
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Aida K
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences University Of Tok
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Kawazoe Ichiro
Department of Aquatic Bioscience, Gradualte School of Agricultural and Life Sciences, The University
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Kawazoe Ichiro
Japan International Research Center for Agricultural Sciences
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Sonobe Haruyuki
Department of Biology, Faculty of Science and Engineering, Konan University
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Nagasawa Hiromichi
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Kawazoe Ichiro
Japan International Research Center For Agriculture Sciences
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Kawazoe I
Univ. Tokyo Tokyo Jpn
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Kawazoe Ichiro
Institute For Research And Development Taiyo Fishery Co. Ltd.
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Ohira T
Kanagawa Univ. Kanagawa Jpn
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Ohira Tsuyoshi
Laboratory Of Genome Science Graduate School Of Marine Science And Technology Tokyo University Of Ma
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Ohira Tsuyoshi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Aida Katsumi
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
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Katayama Akira
Department Of Biochemistry And Molecular Biology Nippon Medical School
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Ohira Tsuyoshi
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Watanabe Toshiki
Ocean Res. Inst. The Univ. Tokyo
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Asahina K
Univ. Utrecht Utrecht Nld
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NISHIMURA Takayuki
Department of Biology, Faculty of Science, Konan University
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OKUNO Atsuro
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Sonobe H
Konan Univ. Kobe Jpn
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Sonobe Haruyuki
Department Of Biophysical Chemistry Kobe Pharmaceutical University
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Ohira Tsuyoshi
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Nagasawa Hiromichi
Department Of Applied Biological Chemistry Graduate School Of Agriculture And Life Sciences Universi
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Nagasawa Hiromichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Okuno Atsuro
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Nishimura T
Biochemical Research Laboratory Ezaki Glico Co. Ltd.
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Sonobe Haruyuki
Department Of Biology Faculty Of Liberal Arts And Science Yamagata University
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長澤 寛道
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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WATANABE Toshiro
Fukuoka Agricultural Research Center
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Nishimura T
Department Of Biochemistry And Molecular Biology Nippon Medical School
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SAITO Hiroyuki
The University of Tokushima
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Nagasawa Hiromichi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Nishimura Takayuki
Department of Biochemistry and Molecular Biology, Nippon Medical School
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