Binding Analysis of Xenopus laevis Translation Initiation Factor 4E (eIF4E) in Initiation Complex Formation.
スポンサーリンク
概要
- 論文の詳細を見る
A translation initiation factor, eIF 4 E, of Xenopus laevis was purified by affinity column chromatography after the gene expression as a full-length protein in a baculovirus-insect cell system. Interaction between X. laevis eIF 4 E and 4 E-BP 2 was analyzed by affinity column chromatography, gel permeation chromatography (GPC), and surface plasmon resonance (SPR). It was found that the interaction of eIF 4 E with an mRNA cap-analogue enhanced the binding activity of eIF 4 E with 4 E-BP 2. Furthermore, the SPR analysis showed that the eIF 4 E cap-analogue interaction was very weak regardless of complex formation of 4 E-BP 2 with eIF 4 E; the dissociation constant of eIF 4 E for the cap-analogue was estimated to be 10-2-10-4 M. These results suggest that the participation of another initiation factor is required for eIF 4 E to recognize the cap structure in vivo. The results reported in this paper support "the performed complex model" of Lee et al., in which eIF 4 E binds to the mRNA cap structure after the initiation factors have formed the initiation complex eIF 4 F.
- 社団法人 日本生化学会の論文
著者
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Nishino Takeshi
Department Of Biochemistry And Molecular Biology Nippon Medical School
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Ishida Toshimasa
Department Of Physical Chemistry Osaka University Of Pharmaceutical Sciences
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IDE Hiroko
Institute for Biomolecular Science, Gakushuin University
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Miyoshi Hiroshi
Institute For Biomolecular Science Gakushuin University
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Wakiyama Motoaki
Institute For Biomolecular Science Gakushuin University
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Hori Hiroyuki
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi
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Okamoto Ken
Department Of Biochemistry And Molecular Biology Nippon Medical School
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Miura Kin-ichiro
Institute For Biomolecular Science Faculty Of Science Gakushuin University
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Ishikawa Masahide
Department of Industrial Chemistry, Faculty of Engineering, The University of Tokyo
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Youtani Takeshi
Departmeat of Physical Chemistry, Osaka University of Pharmaceutical Sciences
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