Substrate Specificity of tRNA (Adenine-1-)-methyltransferase from Thermus thermophilus HB27

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概要

• 論文の詳細を見る

• tRNA (adenine-1-)-methyltransferase was purified to homogeneity from an extreme thermophile, Thermus thermophilus HB27, by several steps of column chromatographies. The molecular weight of this enzyme was about 60, 000 as analyzed by SDS polyacrylamide gel electrophoresis. K_m for E. coli tRNA^<Glu>_2 was 100 nM and that for the methyl group donor, S-adenosyl-L-methionine, was 7. 8 μM. The substrate specificity of the enzyme was investigated by using T7 RNA polymerase transcripts and tRNA fragments obtained by partial digestion with RNases. The enzyme was able to transfer the methyl group to the 3'-half fragment of E. coli initiator tRNA, however, the extent of methylation was elevated by more than five times when the 5'-half fragment was added and annealed to the 3'-half. This indicates that the main recognition site of the enzyme is within the 3'-half region of tRNA molecule, while the tertiary interaction between the T-loop and the D-loop is very effective for the adequate methylation reaction.

• 社団法人日本農芸化学会の論文
• 1994-06-23

著者

• HORI Hiroyuki

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, University of Yamanashi

• Ueda Takuya

  Department Of Radiology Stanford University School Of Medicine

• Ueda T

  Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo

• Watanabe K

  Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University

• WATANABE Kimitsuna

  Department of Chemistry and Biotechnology,Graduate School of Engineering,The University of Tokyo

• Ueda Takuya

  Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo

• Kumagai Izumi

  Department of Biomolecular Engineering, Tohoku University

• OZAWA Kiyoshi

  Department of Biophysical Chemistry, Faculty of Engineering, Yokohama National University

• Nishikawa Kazuya

  Department of Biomolecular Science, Faculty of Engineering, Gifu University

• Yamazaki Norihiko

  Department of Biological Sciences, Faculty of Bioscience and Biotechnology

• Nakanishi Shigeko

  Department of Biophysical Chemistry, Faculty of Engineering, Yokohama National University

• Nakanishi Satoshi

  Strategic Planning Department Pharmaceuticals Company Kyowa Hakko Kogyo Co. Ltd.

• Watanabe Katsunori

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Kumagai Izumi

  Department Of Biochemistry And Engineering Graduate School Of Engineering Tohoku University

• Kumagai Izumi

  Department Of Industrial Chemistry Faculty Of Engineering The University Of Tokyo

• Nishikawa K

  Ochanomizu Univ. Tokyo Jpn

• Hori H

  Department Of Applied Biological Chemistry Tamagawa University

• Ozawa Kiyoshi

  Department Of Bioengineering Faculty Of Engineering Yokohama National University

• Watanabe K

  Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che

• Watanabe K

  Department Of Applied Biological Sciences Faculty Of Agriculture Saga University

• Watanabe K

  Marine Biotechnol. Inst. Iwate Jpn

• Nakanishi S

  Strategic Planning Department Pharmaceuticals Company Kyowa Hakko Kogyo Co. Ltd.

• Hori Hiroyuki

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi

• Ozawa K

  Japan International Res. Center For Agricultural Sci. Okinawa Jpn

• Nishikawa Kazuya

  Department Of Biomolecular Science Faculty Of Engineering Gifu University

• Watanabe Kimitsuna

  Department Of Biological Sciences Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technol

• Yamazaki N

  Univ. Shizuoka Shizuoka Jpn

• Nakanishi S

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Nishikawa Kazuya

  Department of Biological Scinces, Tokyo Institute of Technology

• UEDA Takuya

  Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo

• Ozawa Kiyoshi

  Department of Biochemistry and Molecular Biology, Faculty of Medicine, Nippon Medical School

• WATANABE Kimitsuna

  Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo

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