Synthesis of nonadeca- and octadecaribonucleotides using the solid-phase phosphotriester with tetrahydropyranyl groups as the 2'-hydroxy-protecting group.

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概要

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• Nonadeca- and octadecaribonucleotides corresponding to the D-loop of tRNA<SUP>Phe</SUP> from yeast and the leader sequence of phage f1 coat protein mRNA were synthesized by the activated phosphotriester method. Coupling yield in the synthesis of oligoribonucleotides depended on the extent of nucleosides loaded on controlled pore glass beads (CPG). <I>N</I>-Acyl-5′-<I>O</I>-dimethoxytrityl-2′-<I>O</I>-tetrahydropyranyl derivatives were used as fully protected ribonucleotide monomer units. The 18mer and 19mer corresponding to the D-loop did not serve as substrates for tRNA (guanosine-2′-)methyltransferase from <I>T. thermophilus</I>, but inhibited methylation of the 5′-half fragment of tRNA<SUP>Phe</SUP>. This indicates that both fragments possess some affinity with the enzyme.

著者

• Hirao Ichiro

  Department Of Industrial Chemistry Kyushu Institute Of Technology

• Miura Kin-ichiro

  Department of Industrial Chemistry, Faculty of Engineering, The University of Tokyo

• Hori Hiroyuki

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi

• Ishikawa Masahide

  Department Of Life Chemistry Tokyo Institute Of Technology

• Miura Kin-ichiro

  Department Of Industrial Chemistry Faculty Of Engineering The University Of Tokyo

• Watanabe Kimitsuna

  Department of Industrial Chemistry, Faculty of Engineering, The University of Tokyo

• WATANABE Kimitsuna

  Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo

• MIURA Kin-ichiro

  Department of Applied Chemistry, Faculty of Engineering, University of Tokyo

• Ishikawa Masahide

  Department of Industrial Chemistry, Faculty of Engineering, The University of Tokyo

• Hirao Ichiro

  Department of Chemical Engineering, Kyushu Institute of Technology

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