Expression and Secretion of Bovine β-Lactoglobulin in Saccharomyces cerevisiae(Food & Nutrition)
スポンサーリンク
概要
- 論文の詳細を見る
A bovine β-lactoglobulin (β-LG) was expressed in Saccharomyces cerevlsiae carrying bovine pre-β-LG cDNA and secreted into its growth medium. The expression plasmid was constructed by inserting the whole coding region of the cDNA encoding pre-β-LG between the promoter and terminator of the yeast glyceraldehyde 3-phosphate dehydrogenase gene of pYGlOO, a yeast expression vector. In the supernatant of the yeast growth medium, β-LG with a native conformation was detected by sandwich ELISA, and its amount was estimated to be 1.1 mg/l. A Western-immunoblotting analysis revealed that β-LG secrected in the growth medium had the same mobility as that of authentic bovine β-LG. The N-terminal sequence was also identical with that of authentic mature bovine β-LG.
- 社団法人日本農芸化学会の論文
著者
-
SHIMIZU Makoto
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
-
Kumagai Izumi
Department of Biomolecular Engineering, Tohoku University
-
KAMINOGAWA Shuichi
Department of Food Science and Technology, College of Bioresource Sciences, Nihon University
-
KATAKURA Yoshinori
Department of Genetic Resources Technology, Faculty of Agriculture
-
Kumagai Izumi
Department Of Biochemistry And Engineering Graduate School Of Engineering Tohoku University
-
TOTSUKA Mamoru
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
-
Miura Kin-ichiro
Department of Industrial Chemistry, Faculty of Engineering, The University of Tokyo
-
Totsuka Mamoru
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
-
Katakura Yoshinori
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
-
Miura Kin-ichiro
Department Of Industrial Chemistry Faculty Of Engineering The University Of Tokyo
-
Kaminogawa Shuichi
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
-
Shimizu Makoto
Department Of Applied Biological Chemistry The University Of Tokyo
-
Shimizu Makoto
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
-
MIURA Kin-ichiro
Department of Applied Chemistry, Faculty of Engineering, University of Tokyo
関連論文
- Permeation of Disaccharides Derived from Chondroitin Sulfate through Human Intestinal Caco-2 Cell Monolayers via the Paracellular Pathway
- Integration of PEGylation and refolding for renaturation of recombinant proteins from insoluble aggregates produced in bacteria : Application to a single-chain Fv fragment(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Suppressive Effect of an Isoflavone Fraction on Tumor Necrosis Factor-α-Induced Interleukin-8 Production in Human Intestinal Epithelial Caco-2 Cells
- Cycloheximide Treatment Induces the Uptake of Neutral and Dibasic Amino Acids via the Activation of System b^ in Human Intestinal Caco-2 Cells
- Absorption and Metabolism of Quercetin in Caco-2 Cells
- Milk Fat Globule Membrane Proteins in Aseptically Packed Ultra-heat-treated Milk: Changes during Storage
- INTERACTION OF THE INNER EAR MOTOR PROTEIN PRESTIN WITH CHLORIDE STUDIED BY ISOTHERMAL TITRATION CALORIMETRY(2A1 Micro & Nano Biomechanics IV)
- MISFOLDING AND MISLOCALIZATION BY MUTATIONS OF THE MOTOR PROTEIN PRESTIN(2A1 Micro & Nano Biomechanics IV)
- Effects of Mutation in the Conserved GTSRH Sequence of the Motor Protein Prestin on Its Characteristics(Bioengineering)
- Relationship between Fluorescence Intensity of GFP and the Expression Level of Prestin in a Prestin-Expressing Chinese Hamster Ovary Cell Line(Bioengineering)
- Stable Expression of the Motor Protein Prestin in Chinese Hamster Ovary Cells(Bioengineering)
- Induction of Nitric Oxide Synthase and Subsequent Production of Nitric Oxide Not Involved in Interferon-γ-induced Hyperpermeability of Caco-2 Intestinal Epithelial Monolayers
- Cloning and Expression of the Catalase Gene from the Anaerobic Bacterium Desulfovibrio vulgaris (Miyazaki F)
- Inhibition of P-Glycoprotein Enhances the Suppressive Effect of Kaempferol on Transformation of the Aryl Hydrocarbon Receptor
- Evaluation of Intestinal Dioxin Permeability Using Human Caco-2 Cell Monolayers
- Current Status of Emergency Care for ST-Elevation Myocardial Infarction in an Urban Setting in Japan(Critical Care)
- Age and Growth of Stone Flounder Kareius bicoloratus in Tokyo Bay, Japan
- Cloning and Expression of the Gene Encoding Flavodoxin from Desulfovibrio vulgaris(Miyazaki F)^1
- PJ-867 Impact of the difference of first contact physicians on door-to-balloon time in ST-elevation myocardial infarction(Emergency care / CPR(05)(H),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- Importance of the Hydrogen Bonding Network Including Asp52 for Catalysis, as Revealed by Asn59 Mutant Hen Egg-white Lysozymes
- Complete Nucleotide Sequences of the Genes Encoding Translation Elongation Factors 1α and 2 from a microsporidian parasite, Glugea plecoglossi: Implications for the Deepest Branching of Eukaryotes
- 霧化型励起酸素発生器の基礎研究III
- Effect of Quercetin on the Gene Expression Profile of the Mouse Intestine
- Human CD4^+ central and effector memory T cells produce IL-21 : effect on cytokine-driven proliferation of CD4^+ T cell subsets
- PE-395 The characteristics of the patients with ST-elevation myocardial infarction who achieved a guideline-recommeded door-to-balloon time of 90 minutes(Coronary revascularization, PCI(13)(IHD),Poster Session(English),The 72nd Annual Scientific Meeting o
- OE-394 The present status of emergency care for ST-elevation myocardial infarction in the urban area(Emergency care/CPR(01)(H),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- Effects of changes in leaf height and shoot density on the abundance of two fishes, Rudarius ercodes and Acentrogobius pflaumii, in a Zostera bed
- Stomach contents of the tetraodontid fish, Takifugu pardalis, in Zostera beds at Aburatsubo, central Japan
- Geographic Variation in Growth of the Starspotted Dogfish Mustelus manazo from Five Localities in Japan and Taiwan
- Reproductive Biology of the Starspotted Dogfish Mustelus manazo from Tokyo Bay, Japan
- Age and Growth of the Starspotted Dogfish Mustelus manazo from Tokyo Bay, Japan
- Efficient Construction of a Diabody Using a Refolding System : Anti-Carcinoembryonic Antigen Recombinant Antibody Fragment
- PJ-388 Low/High-density lipoprotein-cholesterol (LDL-C/HDL-C) ratio is the risk factor of coronary artery stenosis(CAS) in the patients with vasospastic angina (VSA)(Angina pectoris, basic/clinical(04)(IHD),Poster Session(Japanese),The 72nd Annual Scienti
- Substrate Specificity of tRNA (Adenine-1-)-methyltransferase from Thermus thermophilus HB27
- Laser-Flash Photolysis in IIigh-Speed Photopolymer Coating Layers Containing Carbonylbiscoumarin Dyes and 2,2'-Bis(2-chlorophenyl)-4,4',5,5' -tetraphenyl- 1 ,1' -bi-1H-imidazole
- Laser Flash Photolysis in High-Speed Photopolymer Coating Layers: Effects of Incorporating Dimethyl Phthalate as a Plasticizer into Coating Formulation
- Laser Flash Photolysis in High-Speed Photopolymer Coating Laysrs
- Acceleration Effects of Phosphine Ligands in the Nickel Catalyzed Methylation of Aldehydes Using Trimethylaluminum
- Regioselective Ring-opening of Cyclopropyl Ketones with Organometallic Reagents
- Catalytic Asymmetric 1, 3-Dipolar Cycloaddition of Nitrile Oxides to an Achiral Allyl Alcohol Utilizing Diisopropyl Tartrate as a Chiral Auxiliary
- Characteristics of Lysophosphatidylcholine in Its Inhibition of Taurine Uptake by Human Intestinal Caco-2 Cells(Food & Nutrition Science)
- Functional Construction of the Anti-Mucin Core Protein (MUCI) Antibody MUSE11 Variable Regions in a Bacterial Expression System
- Titanium Tetraiodide-promoted Tandem Prins Reaction of Alkynes with Acetals : Synthesis of (Z,Z)-1,5-Diiodo-1,3,5-triarylpenta-1,4-dienes
- Transient Up-Regulation of Immunity- and Apoptosis-Related Genes in Caco-2 Cells Cocultured with THP-1 Cells Evaluated by DNA Microarray Analysis
- Detection of various types of human papillomavirus DNA, mainly belonging to the cutaneous-group, more frequently in normal tissue than in squamous cell carcinomas of the lip
- Effect of Neuronal PC12 Cells on the Functional Properties of Intestinal Epithelial Caco-2 Cells(Food & Nutrition Science)
- Production and Characterization of Anti-human Insulin Antibodies in the Hen's Egg(Biological Chemistry)
- Structure of α_-Casein at an Oil/Water Interface: An Analytical Approach Using Immunochemical Methods(Biological Chemistry)
- Methionine Oxidation and Proline cis-trans Isomerization in α_-Casein on Emulsification with Oxidized Lipid(Biological Chemistry)
- Ene Reaction Using the Iminium Salt Generated by the Oxidation of Aminoketene Silyl Aetal
- Mimicry of Erythropoietin and Interleukin-6 Signalling by an Antibody/Cytokine Receptor Chimera in Murine Myeloid 32D Cells
- Crystal Growth Control of ZnO by Anti-ZnO Peptide
- Pathway to Insoluble Aggregates on the Refolding of a Single-chain Fv Antibody : Morphological Changes of Aggregated Protein on Refolding
- Antigen-Mediated Genetically Modified Cell Amplification (AMEGA) with Single Vector Transduction
- Selection of Highly Productive Mammalian Cells Based on an Inducible Growth Advantage Using an Antibody/Receptor Chimera
- A Growth Signal with an Artificially Induced Erythropoietin Receptor-gp130 Cytoplasmic Domain Heterodimer
- Panning of a Phage VH Library Using Nitrocellulose Membranes : Application to Selection of a Human VH Library
- A-3 Selection of Antibody Fragments using Phage Display Library and Preparation of Recombinant Antibody
- Serum and Egg Antibody Responses in Chickens to Escherichia coli(Biological Chemistry)
- Aza-Prins Reaction Promoted by Titanium Tetraiodide and Iodine
- Expression and Secretion of Bovine β-Lactoglobulin in Saccharomyces cerevisiae(Food & Nutrition)
- Unique Occurrence of the 1CF11 Carbohydrate Epitope in Primate Saliva
- Transepithelial Transport of the Bioactive Tripeptide, Val-Pro-Pro, in Human Intestinal Caco-2 Cell Monolayers(Food & Nutrition Science)
- Inhibitory Effect of Arphamenine A on Intestinal Dipeptide Transport
- Exciton Dynamics of Poly(di-n-hexylsilane) Film
- Food and Microhabitat Resource Use by Rudarius ercodes and Ditrema temmincki Coexisting in a Zostera Bed at Aburatsubo, Central Japan
- Lack of increase in postoperative complications with low-dose methotrexate therapy in patients with rheumatoid arthritis undergoing elective orthopedic surgery
- Effects of β-Lactoglobulin on the Tight-junctional Stability of Caco-2-SF Monolayer
- Stabilization of the Tight Junction of the Intestinal Caco-2 Cell Monolayer by Milk Whey Proteins
- Reductive Aldol Reaction of α-Imino Eaters Promoted by Titanium Tetraiodide: Selective Synthesis of α-Amino-β-hydroxyesters
- Human Milk Bile-salt-stimulated Lipase Is Extremely Reactive with the Monoclonal Antibody 1CF11 which Recognizes a Human-specific Carbohydrate Antigen
- Structural Effects of Phenolic Acids on the Transepithelial Transport of Fluorescein in Caco-2 Cell Monolayers(Food & Nutrition Science)
- Sex ratio in the dragonet, Repomucenus valenciennei
- Transepithelial Transport of p-Coumaric Acid and Gallic Acid in Caco-2 Cell Monolayers(Food & Nutrition Sience)
- Emulsifying Properties of Peptides Obtained from the Hydrolyzates of β-Casein(Food & Nutrition)
- Blockade of Salmonella enteritidis Passage across the Basolateral Barriers of Human Intestinal Epithelial Cells by Specific Antibody
- Effects of Carrageenans on the Binding, Phagocytotic, and Killing Abilities of Macrophages to Salmonella(Food & Nutrition Science)
- In vitro Bacteriostatic Effects of Dietary Polysaccharides
- Effect of Quercetin on the Gene Expression Profile of the Mouse Intestine
- Inhibition of P-Glycoprotein Enhances the Suppressive Effect of Kaempferol on Transformation of the Aryl Hydrocarbon Receptor
- Modulation of the Intestinal Ca^ Uptake by a Cheese Whey Protein Digest
- Identification of a Taurine Transport Inhibitory Substance in Sesame Seeds
- Characterization and Regulation of Taurine Transport in Caco-2, Human Intestinal Cells
- Reproductive Cycle and Body Size and Age at Maturity of the Hexagrammid Fish Hexagrammos agrammus in Central Japan
- Effects of Chondroitin Sulfate and Its Oligosaccharides on Toll-Like Receptor-Mediated IL-6 Secretion by Macrophage-Like J774.1 Cells
- A Facile Synthesis of 1β-Methylcarbapenem Skeleton Utilizing Cyclization of α, β-Unsaturated Ester with Methanesulfenyl Chloride
- Hydrogenolysis-Isomerization-Reduction of Propargyl Acetate, and Regio - and Stereoselective Hydrogenation of Dienyl Ester for the Synthesis of 1β-Methylcarbapenem Precursor
- Transepithelial Transport of Ferulic Acid by Monocarboxylic Acid Transporter in Caco-2 Cell Monolayers(Food & Nutrition Science)
- Disappearance of a spring cohort in a population of the dragonet, Repomucenus valenciennei, with spring and autumn spawning peaks in Tokyo Bay, Japan
- The Effect of Hyperosmosis on Paracellular Permeability in Caco-2 Cell Monolayers
- Transient Up-Regulation of Immunity- and Apoptosis-Related Genes in Caco-2 Cells Cocultured with THP-1 Cells Evaluated by DNA Microarray Analysis
- Titanium Tetraiodide-Promoted Reductive Enolate Formation of α-Tosyloxy Ketone Derivatives and Aldol Reaction with Aldehydes
- Daily Increments in the Otolith of Repomucenus valenciennei
- Permeation of Disaccharides Derived from Chohdroitin Sulfate through Human Intestinal Caco-2 Cell Monolayers via the Paracelularl Pathway
- Growth and Life Span of the Hatatatenumeri-dragonet Repomucenus valenciennei in Tokyo Bay, Japan
- Use of 1-Methoxy-1-trimethylsilylallene Oxide and Titanium Tetraiodide as a Dianion Synthon of Methoxyacetone
- Regulation of Diastereofacial Selection in the Allylation Reaction of N-Acyliminium Ion Utilizing Lewis Acids
- Highly Stereodivergent Approach to Both Enantiomers of 4-Substituted β-Lactams via Diastereofacially Controlled Addition of Enolates of t-Butyl Acetate to a Chiral Imine
- A Cation-exchange Resin Promoted Imino Aldol Reaction, Leading to the Synthesis of 2-Isocephem and 2-Oxa-isocephem
- In vivo Insulin Sensitivity and Insulin Binding to Erythrocytes in Children : Insulin Resistance in Obese Children