Effect of Neuronal PC12 Cells on the Functional Properties of Intestinal Epithelial Caco-2 Cells(Food & Nutrition Science)
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概要
- 論文の詳細を見る
The effect of neuronal cells on the functional properties of intestinal epithelial cells was examined by using an in vitro coculture system. Two cell lines, Caco-2 and PC12, were respectively used as intestinal epithelial and enteric neuronal cell models. Coculture of differentiated Caco-2 cells with PC12 caused a significant decrease in the transepithelial electrical resistance (TER) value of the Caco-2 monolayer. The permeability to lucifer yellow (LY) was also significantly increased, suggesting that the tight junction (TJ) of the Caco-2 monolayers was modulated by coculturing with PC12. To identify the TJ-modulating factor presumably secreted from PC12, the effects of the major neurotransmitters on the TER value and LY transport were examined, but no influence was apparent. The TJ-modulating effect of PC12 was prevented by exposing PC12 to cycloheximide, suggesting that new protein synthesis in PC12 was necessary for this regulation.
- 社団法人日本農芸化学会の論文
- 2003-06-23
著者
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SATSU Hideo
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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SHIMIZU Makoto
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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YOKOYAMA Tatsuya
Department of Biotechnology, University of Tokyo
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Satsu Hideo
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Satsu Hideo
Department Of Applied Biological Chemistry Division Of Agricultural And Life Sciences The University
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Yokoyama Tatsuya
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Fujiwara-hatano Yoko
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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OGAWA Nobumasa
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Ogawa Nobumasa
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Fujiwara Yoko
Graduate School Of Humanities And Sciences Ochanomizu Univ.
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Shimizu Makoto
Department Of Applied Biological Chemistry The University Of Tokyo
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Shimizu Makoto
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
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Shimizu Makoto
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Fujiwara Yoko
Department Of Food And Nutritional Science Graduate School Of Humanities And Sciences Ochanomizu University
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FUJIWARA Yoko
Division of Molecular Pharmacology, Department of Pharmacology, Jichi Medical University
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FUJIWARA Yoko
Department of Nutrition and Food Science, Ochanomizu University
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