Analysis of the Comprehensive Effects of Polyunsaturated Fatty Acid on mRNA Expression Using a Gene Chip
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概要
- 論文の詳細を見る
To investigate the comprehensive effects of polyunsaturated fatty acids (PUFA) on gene expression, we analyzed changes of mRNA expression in PUFA-treated HepG2 cells using a DNA micro array. We incubated HepG2 cells for 24h with or without 0.25mM oleic acid (OA), arachidonic acid (AA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA), and then compared the expression profiles of thousands of genes using a GeneChip. PUPA influenced the expression of various genes related to cell proliferation, growth and adhesion, as well as for many transcription factors including sterol regulatory element binding proteins (SREBP). Treatments with AA, EPA, and DHA repressed the expression of genes related to cholesterol and lipid metabolism. Moreover, data from gene chip analysis proved that PUFA reduced the expression of prostasin, which is a serine protease. By measuring the mRNA levels of SREBPs, mevalonate pyrophosphatase and prostasin using quantitative RT PCR, we confirmed the effect of PUFA revealed by gene chip analysis. These data might provide useful clues with which to explore novel functions of PUFA.
- 財団法人 学会誌刊行センターの論文
- 2003-04-01
著者
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Itakura H
Department Of Food Science Ibaraki Christian University
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Itakura Hiroshige
コカコーラ東京研究開発センター
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Itakura Hiroshige
明治製菓
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Itakura H
Ibaraki Christian Univ. Ibaraki Jpn
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Aburatani Hiroyuki
横浜市立大学 大学院医学研究科分子消化管内科学
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Aburatani H
Genome Science Division The University Of Tokyo
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Aburatani Hiroyuki
東京大学 医学系研究科人体病理・病理診断
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Aburatani Hiroyuki
Genome Science Division University Of Tokyo
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Itakura H
National Inst. Health And Nutrition Tokyo Jpn
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Ishii Masami
お茶の水女子大学
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Aburatani Hiroyuki
Division Of Genome Science Research Center For Advanced Science And Technology University Of Tokyo
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YOKOYAMA Masayo
Department of Food Science and Nutrition, Ochanomizu University
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SAWADA Rumi
Department of Food Science and Nutrition, Ochanomizu University
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TSUTSUMI Shunichi
Genome Sciences, Research Center for Advanced Science and Technology, The University of Tokyo
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Ishii M
Univ. Tokyo Tokyo Jpn
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Itakura Hiroshige
Department Of Food Sciences Ibaraki Christian University
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Itakura Hiroshige
Division Of Clinical Nutrition National Institute Of Health And Nutrition
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HANAKA Satoko
Department of Pediatrics, Teikyo University School of Medicine
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Aburatani Hiroyuki
Department Of Genome Science Research Center For Advanced Technology And Science The University Of T
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Aburatani Hiroyuki
Geneme Science Division Research Center For Advanced Science And Technology And Department Of Pathol
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Aburatani Hiroyuki
Genome Science Division Center For Collaborative Research University Of Tokyo
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Itakura H
Division Of Clinical Nutrition National Institute Of Nutrition And Health
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Itakura Hiroshige
Department Of Food Science Ibaraki Christian University
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Ishii Masami
Genome Science Division University Of Tokyo
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Fujiwara-hatano Yoko
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Sawada Rumi
Division Of Medical Devices National Institute Of Health Sciences
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Hanaka Satoko
Division Of Clinical Nutrition National Institute Of Nutrition And Health
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Seyama Y
Department Of Biochemistry Faculty Of Medicine University Of Tokyo:(present)department Of Physiologi
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Seyama Yousuke
Department Of Biochemistry Faculty Of Medicine
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Ishii M
Genome Science Division Research Center For Advanced Science And Technology University Of Tokyo
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Matsumoto Akiyo
Division Of Clinical Nutrition National Institute Of Health And Nutrition
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Fujiwara Yoko
Graduate School Of Humanities And Sciences Ochanomizu Univ.
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Matsumoto Akiyo
Department Of Clinical Dietetics And Human Nutrition Josai University
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Yokoyama Masayo
Department Of Food Science And Nutrition Ochanomizu University
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Tsutsumi Shunichi
Genome Sciences Research Center For Advanced Science And Technology The University Of Tokyo
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Fujiwara Yoko
Department Of Food Science And Nutrition Ochanomizu University
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Fujiwara Yoko
Department Of Food And Nutritional Science Graduate School Of Humanities And Sciences Ochanomizu University
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FUJIWARA Yoko
Division of Molecular Pharmacology, Department of Pharmacology, Jichi Medical University
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FUJIWARA Yoko
Department of Nutrition and Food Science, Ochanomizu University
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Fujiwara Yoko
Department of Food and Nutrition, Ochanomizu University
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