Stereospecificity of hydrogen transfer by pyridine nucleotide-dpendent enoyl reductases in fatty acid synthesis : Studies with enzymes obtained from developing castor bean seeds and Chlorella vulgaris
スポンサーリンク
概要
- 論文の詳細を見る
The mechanism of hydrogen incorporation into fatty acids was investigated with fatty acid synthetase systems from developing castor bean seeds and Chlorella vulgaris. Fatty acids synthesized in the presence of D_2O or stereospecifically deuterium-labeled NADPH or NADH were isolated and analyzed by mass spectrometry to examine the localization of deuterium atoms in the molecule. The stereospecificities of β-ketoacyl-acyl carrier protein (ACP) reductase and enoyl-ACP reductase for reduced pyridine nucleotide were determined with acetoacetyl-ACP and crotonyl-ACP as substrates. The products were also analyzed by gas chromatography-mass spectrometry. The following results were obtained : 1) β-Ketoacyl-ACP reductases from both castor bean seeds and C. vulgaris used the B-side hydrogen of NADPH. 2) Enoyl-ACP reductase from C. vulgaris required NADH for the activity. 3) Enoyl-ACP reductase from castor bean seeds used the A-side hydrogen of NADPH, whereas that from C. vulgaris used the B-side hydrogen of NADH. 4) When stearate was synthesized with the crude fatty acid synthetase fraction from castor bean seeds, hydrogen atoms from water were found on the even-numbered methylene carbon atoms (two hydrogen atoms per carbon atom) and some were found on the odd-numbered methylene carbon atoms. Hydrogen atoms from the B-side of NADPH were found on the odd-numbered methylene carbon atoms (one hydrogen atom per carbon atom). Hydrogen atoms from the A-side of NADPH were also found on the odd-numbered methylene carbon atoms, but the number of incorporated hydrogen atoms was less than expected.
- 日本植物生理学会の論文
著者
-
SEYAMA Yousuke
Department of Food Science and Nutrition, Ochanomizu University
-
Yamada Mitsuhiro
Department of Biology, College of General Education, University of Tokyo
-
Seyama Yousuke
Department Of Biochemistry Faculty Of Medicine
-
Kawaguchi Akihiko
Institute Of Applied Microbiology
-
Saito Kazuki
Institute of Applied Microbiology
-
Okuda Shigenobu
Institute of Applied Microbiology
-
Yamakawa Tamio
Department of Biochemistry, Faculty of Medicine
-
Nakamura Yasunori
Radioisotope Centre
-
Yamakawa Tamio
Department Of Biochemistry Faculty Of Medicine
-
Saito Kazuki
Institute For Biological Resources And Functions Aist
-
Yamada Mitsuhiro
Departmellt Of Biology C:ollegc Of Gencral Educatioll Universsity Of Tokyo
-
Yamada Mitsuhiro
Department Of Biology College Of General Education
関連論文
- A Digital l/f Noise Generator Utilizing Probabilistic Cellular Automata
- Analysis of the Comprehensive Effects of Polyunsaturated Fatty Acid on mRNA Expression Using a Gene Chip
- Influence of Polyunsaturated Fatty Acid on Gene Expression in HepG2 Cells
- Importance of RNA synthesis within the lag phase preceding benzyladenine-induced growth of cucumber cotyledons in the dark
- Glycerolipid synthesis in Avena Leaves during Greening of Etiolated Seedlings III. : Synthesis of α-Linolenoyl-monogalactosyl Diacylglycerol from Liposomal Linoleoyl-phosphatidylcholine by Avena Plastids in the Presence of Phosphatidylcholine-exchange Pro
- Androgenic Control of 1-Alky1-2, 3-Diacylglycerol in the Harderian Gland of the Golden Hamster, Mesocricetus auratus^1
- Stereospecificity of hydrogen transfer by pyridine nucleotide-dpendent enoyl reductases in fatty acid synthesis : Studies with enzymes obtained from developing castor bean seeds and Chlorella vulgaris
- Sexual Dimorphism of Lipids in Harderian Glands of Golden Hamsters
- Transfer of phospholipids from microsomes to mitochondria in germinating castor bean endosperm
- A phosphatidyl choline exchange protein isolated from germinated castor bean endosperms
- Morphological Changes in Woody Stem of Prunus jamasakura under Simulated Microgravity
- Variation of Phosphoenolpyruvate Carboxylase Activity in Dunaliella Associated with Changes in Atmospheric CO_2 Concentration
- Development of Lipid Synthesis from CO_2 in Avena Leaves during Greening of Etiolated Seedlings
- Analytical Studies of Spontaneous and Vascopressin-Induced Calcium Oscillations in Cultured Vasular Smooth Muscle Cells^1
- Induction of Ca^ Oscillations by Vasopressin in the Presence of Tetraethy lammonium Chloride in Cultured Vascular Smooth Muscle Cells^1
- Polar and Neutral Lipid Changes in Spinach Leaves with Ozone Fumigation : Triacylglycerol Synthesis from Polar Lipids
- Change in Levels of Acyl Carrier Proteins in Greening Plants
- Rapid Rehydration and Moderate Plasma Glucose Elevation by Fluid Containing Enzymatically Synthesized Glycogen
- Effect of Thiolactomycin on Lipid Synthesis in Higher Plants
- C-4-3 The Absolute Configuration of (+)-Matrine(Alkaloids and alkaloid biosynthesis)
- In Vivo and In Vitro Positive Interference by Cefpirome in Measurement of Serum Creatinine by the Jaffe Method
- Lipid-Linked Desatuation of Palmitic Acid in Monogalactosyl Diacylglycerol in the Blue-Green Alga (Cyanobacterium) Anabaena variabilis Studied in Vivo
- A Convenient Method for Analyzing Differentially Expressing Genes in Plural Cells
- Sexual Differences in Branched Chain Amino Acid Metabolism into Fatty Acids and Cholesterol in Harderian Gland of Golden Hamster
- General Metabolic Changes in Leaf Lipeds in Response to Ozone : ENVIRONMENTAL AND STRESS RESPONSES
- Effect of Thiolactomycin on Fatty Acid Synthesis in Higher Plants
- Selective Inhibition of Type II Fatty Acid Synthetase by the Antibiotic Thiolactomycin
- Effects of Sesamin and Capsaicin on the mRNA Expressions of 6 and 5 Desaturases in Rat Primary Cultured Hepatocytes
- Glycerolipid synthesis in Avena leaves during greening of etiolated seedlings II. : α-Linolenic acid synthesis
- Glycerolipid Synthesis in Avena Leaves during Greening of Etiolated Seedlings IV. : Effect of Light on Fatty Acid Desaturation
- Glycerolipid synthesis in Avena leaves during greening of etiolated seedlings I. Lipid changes in leaves
- A Measure of Complexity for 1/f Fluctuation
- Short-Time Information Entropy as a Complexity Measure
- Changes in the Level of 7α-Hydroxy-3-oxo-4-cholestenoic Acid in Cerebrospinal Fluid after Subarachnoid Hemorrhage
- Long chain fatty acid synthesis in developing castor bean seeds IV. The synthetic system in proplastids
- Glucose-6-Phosphate Dehydrogenase in Plastids from Developing Castor Bean Seeds
- Long chain fatty acid synthesis in developing castor bean seeds I. The operation of the path from acetate to long chain fatty acids in a subcellular particulate system
- Fatty acid synthesis by spinach chloroplasts III. Relationship between fatty acid synthesis and photophosphorylation
- Analysis of the Comprehensive Effects of Polyunsaturated Fatty Acid on mRNA Expression Using a Gene Chip
- Fatty acid synthesis by spinach chloroplasts I. Property of fatty acid synthesis from acetate
- Fatty acid synthesis by spinach chloroplasts II. The path from PGA to fatty acids
- Long chain fatty acid synthesis in developing castor bean seeds II. Operation of the path from sucrose to long chain fatty acids in a subcellular particulate system
- Lipid Transfer Proteins in Plants and Microorganisms
- Composition of phosphoglycerides and glycosyl glycerides in castor bean mitochondria
- Rapid Rehydration and Moderate Plasma Glucose Elevation by Fluid Containing Enzymatically Synthesized Glycogen
- Effect of Temperature on Starch Degradation in Chlorella vulgaris 11h Cells
- Effects of temperature on glycolate metabolism in Chlorella
- Effects of temperature and CO_2 concentration on photosynthetic CO_2 fixation by Chlorella
- Identification of stereoisomers of methyl hexopyranosides by mass spectrometry of their trifluoroacetates.
- An Electron Spin Resonance Study of the Steroid Conformation –6-Oxohelvolic Acid (Triketone)–
- Genetic regulation of GM2 expression in liver of mouse.
- Induction of Ca2+ Oscillations by Vasopressin in the Presence of Tetraethylammonium Chloride in Cultured Vascular Smooth Muscle Cells