Glycerolipid synthesis in Avena Leaves during Greening of Etiolated Seedlings III. : Synthesis of α-Linolenoyl-monogalactosyl Diacylglycerol from Liposomal Linoleoyl-phosphatidylcholine by Avena Plastids in the Presence of Phosphatidylcholine-exchange Pro
スポンサーリンク
概要
- 論文の詳細を見る
The synthesis of α-linolenoyl-monogalactosyl diacylglycerol (MGDG) by purified Avena plastids from linoleoyl-phosphatidylcholine (PC) was investigated. When Avena plastids were incubated with liposomal [^<14>C]acyl-[^3H]glycerol-PC, the label was transfer-red from the liposomes to the plastids with no change in the ^3H/^<14>C ratio. More than 99% of the label appeared as PC and the remainder as diacylglycerol (DG), MGDG and free fatty acids. The transfer was enhanced by a phosphatidylcholine-exchange protein (PCEP) from germinated castor bean endosperms. Addition of UDP-galactose resulted in the increased synthesis of MGDG. The distribution of ^<14>C among the molecular species of liposomal PC was very low in the hexaene and pentaene species and high in the tetraene and triene species. The labeling pattern of the molecular species was similar for liposomal PC and plastid DG, whereas the ^<14>C in plastid MGDG shifted from the tetraene and triene species toward the hexaene and pentaene species. These results suggest the conversion of liposomal PC to MGDG through DG in Avena plastids and the desaturation of linoleate to α-linolenate on MGDG molecules. In contrast, the major labeled fatty acids were oleic and palmitic acids, when plastids were incubated with [1-^<14>C]acetate, and no further desaturation occurred in each lipid class. This supports our hypothesis that α-linolenoyl-MGDG. is synthesized from the linoleoyl-PC transferred to the plastid from the endoplasmic reticulum by a PCEP, not from oleoyl-MGDG [Ohnishi and Yamada, Plant Cell Physiol. 21: 1607 (1980)].
- 日本植物生理学会の論文
著者
-
Yamada Mitsuhiro
Department of Biology, College of General Education, University of Tokyo
-
Ohnishi Jun-ichi
Department Of Biochemistry Faculty Of Science Saitama University
-
Yamada Mitsuhiro
Departmellt Of Biology C:ollegc Of Gencral Educatioll Universsity Of Tokyo
-
Ohnishi Jun-ichi
Department Of Biochemistry And Molecular Biology Faculty Of Science Saitama University
関連論文
- A Digital l/f Noise Generator Utilizing Probabilistic Cellular Automata
- Importance of RNA synthesis within the lag phase preceding benzyladenine-induced growth of cucumber cotyledons in the dark
- Glycerolipid synthesis in Avena Leaves during Greening of Etiolated Seedlings III. : Synthesis of α-Linolenoyl-monogalactosyl Diacylglycerol from Liposomal Linoleoyl-phosphatidylcholine by Avena Plastids in the Presence of Phosphatidylcholine-exchange Pro
- Stereospecificity of hydrogen transfer by pyridine nucleotide-dpendent enoyl reductases in fatty acid synthesis : Studies with enzymes obtained from developing castor bean seeds and Chlorella vulgaris
- Transfer of phospholipids from microsomes to mitochondria in germinating castor bean endosperm
- A phosphatidyl choline exchange protein isolated from germinated castor bean endosperms
- Morphological Changes in Woody Stem of Prunus jamasakura under Simulated Microgravity
- Development of Lipid Synthesis from CO_2 in Avena Leaves during Greening of Etiolated Seedlings
- Differentiation of Photorespiratory Activity between Mesophyll and Bundle Sheath Cells of C_4 Plants II. Peroxisomes of Panicum miliaceum L.
- Polar and Neutral Lipid Changes in Spinach Leaves with Ozone Fumigation : Triacylglycerol Synthesis from Polar Lipids
- Change in Levels of Acyl Carrier Proteins in Greening Plants
- Proton/Pyruvate Cotransport into Mesophyll Chloroplasts of C_4 Plants : PROTEINS, ENZYMES AND METABOLISM : MEMBRANES AND BIOENERGETICS
- Effect of Thiolactomycin on Lipid Synthesis in Higher Plants
- Two Different Mechanisms for Transport of Pyruvate into Mesophyll Chloroplasts of C_4 Plants a Comparative Study : PROTEINS, ENZYMES AND METABOLISM : MEMBRANES AND BIOENERGETICS
- In Vivo and In Vitro Positive Interference by Cefpirome in Measurement of Serum Creatinine by the Jaffe Method
- Intracellular Localization of Phosphoenolpyruvate Carboxykinase in Bundle Sheath Cells of C_4 Plants
- Differentiation of Photorespiratory Activity between Mesophyll and Bundle Sheath Cells of C_4 Plants I. : Glycine Oxidation by Mitochondria
- Pyruvate Uptake by Mesophyll and Bundle Sheath Chloroplasts of a C_4 Plant, Panicum miliaceum L
- General Metabolic Changes in Leaf Lipeds in Response to Ozone : ENVIRONMENTAL AND STRESS RESPONSES
- Effect of Thiolactomycin on Fatty Acid Synthesis in Higher Plants
- Selective Inhibition of Type II Fatty Acid Synthetase by the Antibiotic Thiolactomycin
- Glycerolipid synthesis in Avena leaves during greening of etiolated seedlings II. : α-Linolenic acid synthesis
- Glycerolipid Synthesis in Avena Leaves during Greening of Etiolated Seedlings IV. : Effect of Light on Fatty Acid Desaturation
- Light-Enhanced Uptake of Metabolites in Mesophyll Protoplasts:Evidence for a Novel pyruvate Transport System
- Glycerolipid synthesis in Avena leaves during greening of etiolated seedlings I. Lipid changes in leaves
- A Measure of Complexity for 1/f Fluctuation
- Short-Time Information Entropy as a Complexity Measure
- Long chain fatty acid synthesis in developing castor bean seeds IV. The synthetic system in proplastids
- Glucose-6-Phosphate Dehydrogenase in Plastids from Developing Castor Bean Seeds
- Long chain fatty acid synthesis in developing castor bean seeds I. The operation of the path from acetate to long chain fatty acids in a subcellular particulate system
- Fatty acid synthesis by spinach chloroplasts III. Relationship between fatty acid synthesis and photophosphorylation
- Fatty acid synthesis by spinach chloroplasts I. Property of fatty acid synthesis from acetate
- Fatty acid synthesis by spinach chloroplasts II. The path from PGA to fatty acids
- Long chain fatty acid synthesis in developing castor bean seeds II. Operation of the path from sucrose to long chain fatty acids in a subcellular particulate system
- Lipid Transfer Proteins in Plants and Microorganisms
- Composition of phosphoglycerides and glycosyl glycerides in castor bean mitochondria
- Characterization of the Plastidic Phosphate Translocators in the Inducible Crassulacean Acid Metabolism Plant Mesembryanthemum crystallinum