Pyruvate Uptake by Mesophyll and Bundle Sheath Chloroplasts of a C_4 Plant, Panicum miliaceum L
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概要
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Intact chloroplasts were isolated from mesophyll and bundle sheath protoplasts of a C_4 plant, Panicum miliaceum L., to measure the uptake of [1^<-14>C]pyruvate into their sorbitol-impermeable spaces at 4℃ by the silicone oil filtering centrifugation method. When incubated in the dark, both chloroplasts showed similar slow kinetics of pyruvate uptake, and the equilibrium internal concentrations were almost equal to the external levels. When incubated in the light, only mesophyll chloroplasts showed remarkable enhancement of the uptake, the internal concentration reaching 10-30 times of the external level after 5 min incubation. The initial uptake rate of the mesophyll chloroplasts was enhanced about ten fold by light and was saturated with increasing pyruvate concentration; K_m and V_<max> were 0.2-0.4mM and 20-40 μmol (mg Chl)^<-1> h^<-1>, respectively. The light enhancement was abolished by DCMU and uncoupling reagents such as carbonylcyanide-m-chlorophenylhydrazone and nigericin. These results indicate the existence of a light-dependent pyruvate transport system in the envelope of mesophyll chloroplasts of P. miliaceum. The uptake activity of mesophyll chloroplasts both in the light and the dark was inhibited by sulfhydryl reagents such as mersalyl and p-chloromercuriphenylsulfonate, but the bundle sheath activity was insensitive to the reagents. These findings are further evidence for the differentiation of mesophyll and bundle sheath chloroplasts of a C_4 plant with respect to metabolite transport.
- 日本植物生理学会の論文
著者
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Ohnishi Jun-ichi
Department Of Biochemistry Faculty Of Science Saitama University
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Kanai Ryuzi
Department Of Biochemistry And Molecular Biology Faculty Of Science Saitama University
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Ohnishi Jun-ichi
Department Of Biochemistry And Molecular Biology Faculty Of Science Saitama University
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