Change in Levels of Acyl Carrier Proteins in Greening Plants

概要

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The levels of acyl carrier proteins (ACP) in greening spinach cotyledons and greening oat leaves were examined by immunoblotting with antiserum raised against spinach ACP I. Two isoforms of ACP, ACP I and ACP II, were found in spinach cotyledons, as they were in the green leaves. The level of ACP II was higher than that of ACP I in etiolated cotyledons. The level of ACP I increased markedly with greening. In the greened cotyledons, the major isoform was ACP I as was the case in green spinach leaves. In oat leaves, two isoforms were also identified, oat ACP I (about 12kDa) and ACP II (about 17kDa), which cross-reacted with the antiserum against spinach ACP I, but which were different from spinach ACPs I and II. The levels of oat ACPs I and II were very low in etiolated leaves. The increase in levels of both ACPs corresponded to the change in the activity of fatty acid synthesis during illumination for 24 h. During further illumination for 24 h, the level of ACP II increased a little in parallel with the change in the activity of fatty acid synthesis, whereas the level of ACP I increased somewhat more. The function of oat ACPs I and II is discussed in connection with the formation of chloroplast.
日本植物生理学会の論文