Isolation and Characterization of a cDNA Clone of UDP-Galactose : Flavonoid 3-O-Galactosyltransferase(UF3GaT) Expressed in Vigna mungo Seedlings

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概要

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• Four cDNA clones were isolated from Vigna mungo seedlings by the screening with cDNA encoding UDP-glucose:flavonoid 3-O-glucosyltransferase(UF3GT) of Antirrhinum majus as a probe; the product of the gene corresponding to one cDNA was more highly expressed in the first simple leaves than in stems. Nucleotide sequence analysis revealed 1, 691 bp (including 326 bp non-reading) containing an open reading frame of 455 amino acids. The deduced amino acid sequence showed 42% and 23% identity with those of A. majus UDP-glucose:flavonoid 3-O-glucosyltransferase(UF3GT) and Petunia hybrida UDP-rhamnose:anthocyanidin 3-O-glucoside rhamnosyltrans-ferase(RT), respectively. One region of the cDNA(amino acids 325 to 387) showed similarity to ceramide UDP-galactosyltransferases of mice, rats and humans. A crude extract from Escherichia coli, in which the protein was expressed from the cDNA, showed high UF3GaT activity but low UF3GT activity, and was similar in K_m, optimal pH and substrate specificity to UF3GaT from V.mungo. We conclude that we have obtained UDP-galactose:flavonoid 3-O-galactosyltransferase(UF3GaT) cDNA from V.mungo.

• 日本植物生理学会の論文

著者

• OZEKI Yoshihiro

  Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agricultur

• Aida R

  National Institute Of Floricultural Science

• Aida Ryutaro

  Natl. Res. Inst. Of Veg. Ornam. Plts. & Tea

• Itoh Yoshio

  Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology

• Shibata Michio

  Department of Internal Medicine, Tokyo Medical College Hospital

• TERAMOTO Susumu

  Department of Biological Science, Faculty of Science, Kumamoto University

• YOSHITAMA Kunijiro

  Department of Biological Science, Faculty of Science, Kumamoto University

• Shibata Michio

  Natl. Res. Inst. Of Veg. Ornam. Plts. & Tea

• Shibata Michio

  Department Of Internal Medicine Tokyo Medical College Hospital

• Ito Y

  National Inst. Agrobiological Sci. Ibaraki Jpn

• Ozeki Yoshihiro

  Dept. Biotech. Fac. Tech. Tokyo Univ. Agri. & Tech.

• Ozeki Yoshihiro

  Department Of Biology College Of Arts And Science The University Of Tokyo

• Ito Yoko

  Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology

• Shibata Masaru

  Natl. Res. Inst. Of Veg. Ornam. Plts. & Tea

• Yoshitama Kunijiro

  Fac. Sci. Kumamoto Univ.

• Yoshitama Kunijiro

  Department Of Biological Science Faculty Of Science Kumamoto University

• Mato Masami

  Department of Floriculture, National Research Institute of Vegetables, Ornamental Plants and Tea

• Higeta Daisuke

  Department of Biotechnology, Tokyo University of Agriculture and Technology

• Aida Ryutaro

  Department of Floriculture, National Research Institute of Vegetables, Ornamental Plants and Tea

• TERAMOTO Susumu

  Fac. Sci. Kumamoto Univ.

• Mato Masami

  Natl. Res. Inst. Of Veg. Ornam. Plts. & Tea

• Mato Masami

  Department Of Biological Science Faculty Of Science Kumamoto University

• Higeta Daisuke

  Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology

• Ozeki Y

  Univ. Tokyo Tokyo Jpn

• Teramoto Susumu

  Department Of Aeronautics And Astronautics School Of Enginnering The University Of Tokyo

• Ozeki Y

  Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology

• Itoh Yoshio

  Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology

• Itoh Yoshio

  Department of Applied Chemistry, Faculty of Engineering, Tohoku University

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