Efficient Transgene Expression in Chrysanthemum, Dendranthema grandiflorum (Ramat.) Kitamura, by Using the Promoter of a Gene for Chrysanthemum Chlorophyll-a/b-binding Protein

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概要

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• We investigated the usefulness of the promoter of a gene for chrysanthemum chlorophyll-a/b-binding protein (Cab) for transgene expression in the chrysanthemum Dendranthema grandiflorum (Ramat.) Kitamura. We used the promoter region of a Cab gene isolated from the chrysanthemum-wild species D. japonicum Makino. The 35S promoter of cauliflower mosaic virus (CaMV) or the Cab promoter was fused to the β-glucuronidase gene (gus) and introduced into the chrysanthemum. We obtained 300 putative transformants (115 with 35S/gus and 185 with Cab/gus). GUS assay of the leaves of the in vitro plants revealed that 9.6% (11/115) of the putative plants to which 35S/gus had been introduced and 24.3% (45/185) of the putative plants to which Cab/gus had been introduced were GUS-positive. Southern blot analysis showed that even the GUS-negative plants harbored the gus gene in their genomes. The Cab promoter expressed the transgene more efficiently than the 35S promoter and could be used for transgene expression in chrysanthemum leaf tissues.

• 日本育種学会の論文
• 2004-03-01

著者

• Ohmiya Akemi

  National Institute Of Floricultural Science

• Kishimoto Sanae

  National Institute Of Floricultural Science Fujimoto

• Kishimoto Sanae

  National Institute Of Floricultural Science

• Aida Ryutaro

  National Institute Of Floricultural Science National Agriculture And Food Research Organization

• Aida R

  National Institute Of Floricultural Science

• Aida Ryutaro

  Natl. Res. Inst. Of Veg. Ornam. Plts. & Tea

• Aida Ryutaro

  National Institute Of Floricultural Science National Agricultural Research Organization

• Yoshida K

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Yoshida Kazuya

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• TANAKA Yoshikazu

  Institute for Geothermal Sciences Graduate School of Science, Kyoto University

• OHIRA Kazuyuki

  Institute for Advanced Research, Suntory, Ltd.

• Shibata Michio

  National Institute Of Floricultural Science:graduate School Of Life And Environmental Sciences Unive

• Shibata M

  National Institute Of Floricultural Science

• Shibata Michio

  National Institute Of Floricultural Science National Agricultural Research Organization

• Ohmiya A

  National Institute Of Floricultural Science

• Ohira Kazuyuki

  Institute For Advanced Technology Suntory Ltd.:(present Address) Intellectual Property Devision Nara

• Aida Ryutaro

  National Institute Of Floricultural Science

• Tanaka Yoshikazu

  Institute For Advanced Technology Suntory Ltd.

• Tanaka Yoshikazu

  Institute For Advanced Core Technology

• Shibata Michio

  National Institute of Floricultural Science

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