Isolation of Daucus carota ethylene insensitive3-like (DcEIL) involved in stress-inducible DcMYB1 expression in suspension-cultured carrot cells
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概要
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In carrot suspension-cultured cells, the expression of phenylalanine ammonia-lyase 1 (DcPAL1) gene was crucially regulated by the transcription regulatory factor Daucus carota MYB1 (DcMYB1). To elucidate the regulatory mechanism of DcMYB1 expression, we isolated and identified the transcription regulatory factor D. carota ethylene-insensitive3 (EIN3)-like protein (DcEIL) from a cDNA library prepared from suspension-cultured carrot cells treated with the “dilution effect” using a yeast one-hybrid system. DcEIL bound to a region of the DcMYB1 promoter containing a putative cis-element, which suppressed DcMYB1 promoter expression. The amino acid sequence of DcEIL contained the predicted signal sequence for nuclear localization, and transportation to the nucleus was confirmed using a green fluorescent protein-DcEIL fusion protein expressed in suspension-cultured Arabidopsis thaliana cells. The DcEIL gene was constitutively expressed irrespective of the elicitor treatment or the dilution effect. These results suggest that the binding of DcEIL to a cis-element of the DcMYB1 promoter might be regulated at the posttranslational level as a consequence of the regulation of DcPAL1 gene expression via DcMYB1 expression.
- 2010-03-25
著者
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SASAKI Nobuhiro
Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agricultur
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OZEKI Yoshihiro
Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agricultur
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Kimura Soichi
Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agricultur
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MIYAHARA Taira
Department of Biotechnology, Tokyo University of Agriculture and Technology
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SATOH Shunsuke
Department of Biotechnology, Tokyo University of Agriculture and Technology
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MAEDA Kazuhiro
Department of Biotechnology, Tokyo University of Agriculture and Technology
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Kimura Soichi
Department Of Biotechnology Tokyo University Of Agriculture And Technology
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Miyahara Taira
Department Of Biotechnology Tokyo University Of Agriculture And Technology
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Maeda Kazuhiro
Department Of Biotechnology Tokyo University Of Agriculture And Technology
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Ozeki Yoshihiro
Department Of Biotechnology Tokyo University Of Agriculture And Technology
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Ozeki Yoshihiro
Department Of Biology College Of Arts And Science The University Of Tokyo
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Satoh Shunsuke
Department Of Biotechnology Tokyo University Of Agriculture And Technology
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Ozeki Yoshihiro
Department Of Biotechnology And Life Science Faculty Of Engineering Tokyo University Of Agriculture
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Sasaki Nobuhiro
Dep. Of Biotechnology Tokyo Univ. Of Agriculture And Technol.
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Ozeki Yoshihiro
Department Of Biotechnology And Life Science Faculty Of Engineering Tokyo University Of Agriculture
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Maeda Kazuhiro
Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology
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Sasaki Nobuhiro
Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology
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MAEDA Kazuhiro
Department of Bioscience and Bioinformatics, Kyushu Institute of Technology
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