Cloning and heterologous expression of cDNAs encoding flavonoid glucosyltransferases from Dianthus caryophyllus
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概要
- 論文の詳細を見る
Yellow petals of carnations contain chalcone 2′-O-glucoside. The glucosylation occurs after the p-coumaroyl CoA and malonyl-CoA condensation reaction by chalcone synthase (CHS), but the enzyme(s) transferring glucose from UDP-glucose to the 2′-OH position of chalcone has not been identified. The full-length cDNA clones for 18 glucosyltransferase (GT) genes were isolated from petal tissue of carnation (Dianthus caryophyllus) bearing various flower colors. The 18 GTs encoded in the cDNAs were enzymatically characterized in an E. coli expression system using chalcone, flavanone, flavone, flavonol and anthocyanidin as substrates. Three of the 18 were characterized as 3-GT possessing different substrate specificities for flavonoids and anthocyanidin and another two GTs catalyzed the transfer of glucose to the 2′-hydroxyl group of chalcone. In addition, these two enzymes glucosylated flavonol (3-OH and 7-OH), flavanone (7-OH), flavone (7-OH) and anthocyanidin (3-OH and 7-OH).
- 日本植物細胞分子生物学会の論文
- 2004-12-01
著者
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OZEKI Yoshihiro
Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agricultur
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Itoh Yoshio
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
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Ogata Jun
Department Of Preventive Medicine National Cardiovascular Center
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Ogata Jun
Department Of Biological Science Faculty Of Science Kumamoto University
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Ito Y
National Inst. Agrobiological Sci. Ibaraki Jpn
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Ozeki Yoshihiro
Dept. Biotech. Fac. Tech. Tokyo Univ. Agri. & Tech.
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Ozeki Yoshihiro
Department Of Biology College Of Arts And Science The University Of Tokyo
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Ogata Jun
Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology
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Ito Yoko
Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology
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ISHIDA Madoka
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
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YOSHIDA Hiroyuki
Japan Tobacco Inc. Applied Plant Research Center
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Ishida Madoka
Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology
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Ozeki Y
Univ. Tokyo Tokyo Jpn
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YOSHIDA Hiroyuki
JT・APRL
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Ozeki Y
Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology
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Yoshida Hiroyuki
Japan Atomic Energy Agency Nuclear Science And Engineering Directorate
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Itoh Yoshio
Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology
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Yoshida Hiroyuki
Japan Atomic Energy Agency
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Itoh Yoshio
Department of Applied Chemistry, Faculty of Engineering, Tohoku University
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