Changes in Activities of Enzymes Involved in General Phenylpropanoid Metabolism during the Induction and Reduction of Anthocyanin Synthesis in a Carrot Suspension Cultue as Regulated by 2,4-D

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The activities of enzymes involved in general phenylpropanoid metabolism were followed in a carrot suspension culture during the induction and reduction of anthocyanin synthesis regulated by 2,4-D. When no anthocyanin synthesis occurred in a medium containing 2,4-D (+2,4-D medium), the activities of phenylalanine ammonia-Iyase (PAL) and 4-coumarate:CoA ligase (4CL) increased I day after transfer due to the transfer effect, but subsequently decreased and remained at a low level. Cinnamate-4-hydroxylase (C4H) activity showed a low level throughout culture. When cells were transferred to a medium lacking 2,4-D (-2,4-D medium), the activities of PAL, C4H and 4CL increased and maximum activities of these enzymes were observed 6-7 days after transfer, when anthocyanin was most rapidly synthesized. When cells were cultured in the -2,4-D medium, the addition of 2,4-D immediately reduced the induced activity of PAL. PAL activity was super-induced by the transfer effect, while anthocyanin synthesis decreased. The addition of intermediates of general phenylpropanoid metabolism, with 2,4-D, to the medium 6 days after transfer to the -2,4-D medium did not promote anthocyanin synthesis, whereas dihydroquercetin did promote it. Regulation of anthocyanin synthesis by 2,4-D is discussed in relation to changes in enzyme activities involved in general phenylpropanoid metabolism.
日本植物生理学会の論文