Structure and Expression of Chalcone Synthase Gene in Carrot Suspension Cultured Cells Regulated by 2,4-D

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概要

• 論文の詳細を見る

• Two nearly full-length chalcone synthase (EC 2.3.1.74) cDNAs, designated CHS2 and CHS9, were isolated from a cDNA library of mRNA prepared from anthocyanin-synthesizing cells of carrot suspension cultures and showed 98% nucleotide sequence identity to each other. The genomic sequence (gCHS2), which corresponds to CHS2, was isolated from a carrot genomic library. Primer extension identified the transcription start site of gCHS2 at 72 bp upstream from the initiation codon ATG, and showed that gCHS2 was activated during anthocyanin synthesis. A chimeric gene carrying the gCHS2 putative promoter sequence from - 2,357 bp to +92 bp connected to the firefly luciferase gene and Nos terminator was introduced by electroporation into protoplasts prepared from anthocyanin-synthesizing carrot cells and the effects of light and 2,4-D on the promoter activity were examined by a transient assay for luciferase activity. Luciferase activity was induced by light exposure in the medium lacking 2,4-D but suppressed by the addition of 2,4-D and/or dark treatment.

• 日本植物生理学会の論文

著者

• OZEKI Yoshihiro

  Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agricultur

• Takeda Junko

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

• Ozeki Yoshihiro

  Department Of Biology College Of Arts And Sciences The University Of Tokyo

• Ozeki Yoshihiro

  Department Of Biology College Of Arts And Science The University Of Tokyo

• Davies Eric

  School Of Biological Sciences University Of Nebraska-lincoln:(present Address)botany Department Nort

• Davies Eric

  School Of Biological Sciences University Of Nebraska-lincoin

• Davies Eric

  School Of Biological Sciences University Of Nebraska

• Takeda Junko

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University

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