Quantitative Isolation of Undegraded Polysomes from Aged Pea Tissue in the Absence of Contaminants and Artefacts

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概要

• 論文の詳細を見る

• Undegraded polysomes were isolated successfully from aged pea epicotyls by grinding frozen tissue in at least 10 volumes of buffer A (0.2 M Tris-HCl, pH 8.5; 0.2 M sucrose; 60 mM KCl; 30 mM MgCl_2). taking care to prevent the tissue from thawing prior to homogenization. Supposedly pure polysomes, derived from the membrane-bound polysome fraction, were apparently contaminated with membranes, and contained polysomes clumped together via nascent chains. Problems with contaminants and artefacts were partially alleviated by the use of polyoxyethylene tridecyl ether as a detergent replacing Triton X-100; further alleviated by the use of large volumes of detergent-containing buffer to resuspend the membrane-bound polysome; and almost completely eliminated by brief treatment of resuspended polysomes with protease K. Optimal conditions for isolating polysomes from aged tissue are given.

• 日本植物生理学会の論文

著者

• Davies Eric

  School Of Biological Sciences University Of Nebraska-lincoln:(present Address)botany Department Nort

• Davies Eric

  School Of Biological Sciences University Of Nebraska

• Abe Shunnosuke

  School Of Biological Sciences University Of Nebraska:(present)institute Of Agricultural Environment

関連論文

• Wounding of Aged Pea Epicotyls Enhances the Reinitiating Ability of Isolated Ribosomes
• Wounding Inhibits Protein Synthesis yet Stimulates Polysome Formation in Aged, Excised Pea Epicotyls
• Structure and Expression of Chalcone Synthase Gene in Carrot Suspension Cultured Cells Regulated by 2,4-D
• Quantitative Isolation of Undegraded Polysomes from Aged Pea Tissue in the Absence of Contaminants and Artefacts
• The Wound Response in Tomato Involves Rapid Growth and Electrical Responses, Systemically Up-Regulated Transcription of Proteinase Inhibitor and Calmodulin and Down-Regulated Translation

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