Changes in the Activities of Various Glycosidases during Carrot Cell Elongation in a 2,4-D-Free Medium
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概要
- 論文の詳細を見る
Changes in the activities of some glycosidases were studied in carrot suspension cultures with and without 2,4-D. Remarkable cell elongation occurs in a medium without 2,4-D, while few cells elongate in a medium containing it. Glycosidases were fractionated into soluble, ionically wall-bound, tightly wall-bound and extracellular enzymes. The optimum pHS of all the ionically bound glycosidases were in an acidic range, 4.4-5.0. The activities of the ionically and tightly bound β-xylosidases and β-galactosidases were higher in elongating than in non-elongating cells. Furthermore, the activities of these enzymes increased with cell elongation during culture, suggesting that they may play important roles in cell elongation. Higher activities of soluble and cell wall-bound β-glucosidase and α-mannosidase were found in non-elongating rather than in elongating cells. The activities of all soluble glycosidases except β-xylosidase were also higher in non-elongating cells. Only β-xylosidase and β-galactosidase activities were detectable in the medium of the elongation culture.
- 日本植物生理学会の論文
著者
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MASUDA Hiroshi
Department of Fermentation Technology, Yamanashi University
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Ozeki Yoshihiro
Department Of Biology College Of Arts And Science The University Of Tokyo
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Ozeki Yoshihiro
Department Of Botany Faculty Of Science University Of Tokyo
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Masuda H
Hitachi Ltd. Tokyo Jpn
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Amino Shin-ichi
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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Komamine Atsushi
Department Of Botany Faculty Of Science The University Of Tokyo:(present)biological Institute Facult
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Komamine Atsushi
Department Of Botany Faculty Of Science University Of Tokyo:(present)biological Institute Tohoku Uni
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Amino Shin-ichi
Department Of Botany Faculty Of Science University Of Tokyo
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Amino Shin-ichi
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Masuda Hiroshi
Department Of Agricultural Chemistry Obihiro University Of Agriculture And Veterinary Medicine
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