Detection of 25-kDa Anti-Agglutinin in Epididymal Plasma and Spermatozoa Collected from Various Regions of Boar Epididymis
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概要
- 論文の詳細を見る
Distribution of the 25-kDa epididymal anti-agglutinin for spermatozoa was examined by the Western blotting technique in fluid collected from boar rete testes and epididymides. Fluid was collected from the rete testes and various regions of the epididymis, and then plasma and sperm extracts were prepared. Antiserum was raised against the anti-agglutinin, and specific antibody was purified from it. In the plasma, a reaction between the anti-agglutinin and purified antibody was first observed in the proximal corpus epididymidis and became stronger in the more distal regions. In extracts from washed spermatozoa using the original sample buffer, anti-agglutinin was detected between the proximal corpus and the distal cauda and more strongly in the distal corpus. A thorough washing of spermatozoa on a discontinuous Percoll gradient brought about only minor changes in the detection pattern of anti-agglutinin. However, in samples extracted from washed spermatozoa using a modified sample buffer containing twofold detergent and twofold 2-mercaptoethanol, a stronger reaction was observed between the anti-agglutinin and purified antibody in the distal cauda than in the distal corpus. These results indicate that free or loosely sperm-bound anti-agglutinin increases in the corpus and cauda epididymides. It is also suggested that solubility of the anti-agglutinin associated with spermatozoa in the original sample buffer decreases in the cauda epididymidis.
- 日本繁殖生物学会の論文
- 1995-05-01
著者
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MASUDA Hiroshi
Department of Fermentation Technology, Yamanashi University
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MIYANO Takashi
Department of Animal Sciences, Kobe University
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Miyake Masashi
Department Of Human Genetics School Of International Health The University Of Tokyo
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Masuda Hiroshi
Lab.anim.sci.& Toxicol.labs.sankyo Co. Ltd.
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MIYANO Takashi
Animal Reproduction and Biotechnology, Graduate School of Agricultural Science, Kobe University
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Masuda H
Obihiro Univ. Agriculture And Veterinary Medicine Hokkaido Jpn
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Harayama Hiroshi
Graduate School Of Agricultural Sci. Kobe Univ.
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Miyano T
Animal Reproduction And Biotechnology Graduate School Of Agricultural Science Kobe University
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Miyano Takashi
Laboratory Of Reproductive Biology & Biotechnology Faculty Of Agriculture Kobe University
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HARAYAMA Hiroshi
Division of Bioscience, Graduate School of Science and Technology, Kobe University
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MIYAKE Masashi
Division of Bioscience, Graduate School of Science and Technology, Kobe University
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KATO Seishiro
Department of Animal Science, Faculty of Agriculture, Kobe University
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Masuda H
Department Of Biosciences And Technology Faculty Of Agriculture Iwate University
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Miyano T
Laboratory Of Reproductive Biology And Biotechnology Faculty Of Agriculture Kobe University
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Kato S
Department Of Animal Science Faculty Of Agriculture Kobe University
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Kato Seishiro
Department Of Animal Science Faculty Of Agriculture Kobe University
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Masuda Hiroshi
Department Of Agricultural Chemistry Obihiro University Of Agriculture And Veterinary Medicine
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MIYANO Takashi
Department of Animal Reproduction, Faculty of Agriculture
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