Lectin-Binding Characteristics of Extracts from Epididymal Boar Spermatozoa
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概要
- 論文の詳細を見る
Glycosylated components of boar spermatozoa and epididymal plasma from the central caput, distal corpus and distal cauda epididymides were compared by electrophoretic and lectin blotting techniques. The four lectin reagents used in this study were Concanavalin A (Con A), Lens Culinaris Agglutinin (LCA), Ricinus Communis Agglutinin I (RCA I) and Peanut Agglutinin (PNA), which were labeled with peroxidase. The affinity of Con A and of LCA increased for three bands and one smear (A: > 86.8 kDa, B: 80 kDa, C: 65-75 kDa and D: 45 kDa) and for two bands and one smear (A, B and C) of extracts from spermatozoa, respectively, as they passed through the epididymis. RCA I apparently recognized one band and one smear (G: > 86.8 kDa and H: 46-58 kDa) of extracts from the distal cauda spermatozoa, though it hardly or faintly reacted with those from the central caput and distal corpus spermatozoa. PNA showed a similar affinity for band "G" to RCA I. Moreover, bands and smears with similar mobility (A, B, C, D, G and H) were also detected in epididymal plasma by lectin blotting techniques. On the other hand, several sperm components were detected more visibly with Con A (E: 37-41 kDa and F: 32-37 kDa), RCA I (I: 41 kDa) and PNA (I: 41 kDa and J: 47-51 kDa) in sperm extracts from the distal cauda than in those from the central caput, though no corresponding components were observed in epididymal plasma. These epididymal maturation-dependent modifications related to glycosylation in boar spermatozoa are discussed.
- 日本繁殖生物学会の論文
- 1998-02-01
著者
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MASUDA Hiroshi
Department of Fermentation Technology, Yamanashi University
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Kannan Y
Kobe Univ. Kobe Jpn
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Miyake Masashi
Department Of Human Genetics School Of International Health The University Of Tokyo
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Masuda Hiroshi
Lab.anim.sci.& Toxicol.labs.sankyo Co. Ltd.
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Masuda H
Obihiro Univ. Agriculture And Veterinary Medicine Hokkaido Jpn
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Harayama Hiroshi
Graduate School Of Agricultural Sci. Kobe Univ.
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HARAYAMA Hiroshi
Division of Bioscience, Graduate School of Science and Technology, Kobe University
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MIYAKE Masashi
Division of Bioscience, Graduate School of Science and Technology, Kobe University
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KATO Seishiro
Department of Animal Science, Faculty of Agriculture, Kobe University
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Masuda H
Department Of Biosciences And Technology Faculty Of Agriculture Iwate University
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KANNAN Yasuyuki
Department of Animal Science, Faculty of Agriculture, Kobe University
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WATANABE Shin-ya
Department of Animal Reproduction, National Institute of Animal Industry
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Kannan Yasuyuki
Department Of Animal Science Faculty Of Agriculture Kobe University
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Kato S
Department Of Animal Science Faculty Of Agriculture Kobe University
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Watanabe Shin-ya
Department Of Animal Reproduction National Institute Of Animal Industry
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Kato Seishiro
Department Of Animal Science Faculty Of Agriculture Kobe University
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Masuda Hiroshi
Department Of Agricultural Chemistry Obihiro University Of Agriculture And Veterinary Medicine
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